Effect Of Trimetazidine Preconditioning On Ischemia-Reperfusion Injury Of Rat Cardiomyocyte Apoptosis And Expression Of Mn-SOD

Background and PurposeMyocardial ischemia-reperfusion injury(MIRI)refers to the ischemic cardiovascular disease in early coronary artery percutaneous catheter(PTCR),percutaneous transluminal coronary angioplasty(PTCA),coronary artery bypass grafting(CABG),orthotopic heart transplant(OHT)and reperfusion therapy were more prone to may cause myocardial tissue a common clinical symptom of further damage.The etiopathogenesis of MIRI is intricacy,in which cell apoptosis,thanatosis of myocardial cells & the generation of oxygen free radicals are thought of the primary reason of the bruise progress.Nowadays,the study on MIRI is a hot topic in clinic.This study aims to establish a rat model of myocardial ischemia/reperfusion injury to observe the effects of trimetazidine preconditioning on the expressions of apoptosis-related genes Bcl-2,Bax,Caspase-3 and superoxide dismutase(SOD)and malondialdehyde(MDA)in myocardial cells of MIRI rats,and to explore the possible protective mechansm.Materials & methods30 healthy 8-week-old male SD rats were shuffle grouping into bogus action group(group a,n=10),MIRI patterns group(group b,n=10),and TMZ pre lavage group(group c,n=10).By etiopathogenesis the histopathology alter of atrial tissue,the thanatosis of myocardial cells was generated,and the expression levels of CK,CK-MB,SOD,MDA and Bcl-2,Bax,Caspase-3,in serum were tested.Consequence1.The cell and tissue structure of group A were basically normal.2.In group B,the swelling of myocardial cells was the most obvious and the arrangement of myocardial fibers was disordered.3.Compared with group B,the swelling of cardiac myocytes in group C was significantly reduced,and the apoptosis index was also significantly decreased(P<0.01).Serum enzymology index:Compared with group B,SOD activity(84.21± 6.07 ug /L)and Bcl-2 m RNA(3.12±1.86)of SD rats in group C were significantly increased(P<0.01).Malondialdehyde(MDA)was 33.58±3.73 mmol/L.The creatine kinase(CK)was177.93±5.11 U/L.The creatine kinase isoenzyme(CK-MB)was 50.92±2.94 U/L.The m RNA expression levels of Bax and Caspase-3 were also significantly decreased(2.41±0.19,2.34±0.23).In addition,the differences among the experimental results of group A,B and C were statistically significant(P<0.01).ConclusionTrimetazidine can reduce the contents of MDA,CK,and CK-MB by increasing the activity of SOD.The expression level of Bcl-2 m RNA was up-regulated,and the expr-ession levels of Bax and Caspase-3 m RNA were decreased,which effectively inhibite-d the apoptosis of myocardial cells in MIRI model rats,and had a certain protective effect on myocardial cells.