Study On Process Optimization And Quality Standard Of Rongshu Oral Liquid Extract

Objective:To study the optimum process conditions of horizontal spiral continuous dynamic countercurrent extraction of Rongshu oral liquid extract,and establish the quality standard of Rongshu oral liquid,so as to provide basis for quality control and evaluation of oral liquid.Methods:（1）Using single factor and L₉（3⁴）orthogonal experimental design,set up by HPLC determination of ferulic acid and pinot content and extract yield comprehensive score as an index,crushing degree of horizontal spiral continuous dynamic countercurrent extraction,solvent ratio,extraction time,extraction temperature,four factors,the best water extraction process was optimized.（2）With ferulic acid and the retention rate of pinot element and extract the lower rate as the index,more chitosan clarification method,centrifugation,three different ways in the alcohol sinking method,select out the best in the process.（3）Study the forming process of rongshu oral liquid.（4）TLC method was used for qualitative identification of 5 medicinal herbs in Rongshu oral liquid.（5）By high performance liquid chromatography（HPLC）method for the preparation of the ferulic acid content determination and si pinault,chromatographic conditions:Ultimate XB-C₁₈（GD）（4.6 mm×250 mm,5μm）,acetonitrile-0.1%acetic acid（13:87）,the flow rate is 1 ml/min,column temperature 30℃,the detection wavelength of 330 nm;sample volume 10μl.Results:（1）The best water extraction conditions were:35,the degree of comminution was 10,the extraction time was 90 min,and the extraction temperature was 65℃.（2）The impurity removal process was concentrated at 60℃,concentrated to 1:0.5 and ml at 15 min 3000 r/min.（3）When the flavoring agent is honey,the dosage is 15 ml,and the taste is better.The preservative was determined to be 0.2%sorbic acid.（4）The TLC identification method of Angelica sinensis,Zizyphus jujuba kernel,rose,Lycium barbarum and Schisandra chinensis in oral liquid was established.（5）By high performance liquid content determination of ferulic acid in linear relationship between oral liquid chromatography and spinosin good,good stability,good reproducibility.Conclusion:（1）It can save solvent and shorten extracting time by using horizontal horizontal spiral continuous dynamic counter-current extraction technology,and provide important parameters for pharmaceutical factory.（2）The liquid phase can be used for the determination of content determination of ferulic acid in Rongshu oral liquid and spinosin.