Structure Characterization And Biological Activities Of Polysaccharide From Chroogomphis Rutilus

As a precious undergrowth resource,Chroogomphis rutilus had high medicinal value.The polysaccharide extracted from its fruit body is a kind of macromolecular biopolymer.Due to the diversity of its connection mode and conformation,it was endowed with polysaccharide Many biological functions,such as antioxidant activity,immunomodulatory activity,anti-tumor activity,hypoglycemic activity,etc.Separation and purification was a key link in the study of the structure and characterization of polysaccharides,because the biological activity of polysaccharides were closely related to its structure.Therefore,this article used microwave extraction method to extract the polysaccharides from Chroogomphis rutilus,optimizes the extraction rate and active extraction process of the polysaccharides by response surface method,and then separated and purified the polysaccharides from Chroogomphis rutilus to analyze the structure of the polysaccharides.At the same time,the antioxidant activity and immunomodulatory activity of the isolated and purified polysaccharide were analyzed,and the structure-activity relationship of the polysaccharide was explored.The research results were as follows:(1)The microwave extraction method was used to extract the polysaccharides of Chroogomphis rutilus,and the polysaccharides extraction rate and polysaccharides activity were optimized by response surface.The microwave extraction method had the advantages of short extraction time and high extraction rate.At the same time,research had shown that the microwave extraction of Chroogomphis rutilus.The antioxidant activity and immunomodulatory activity of mushroom polysaccharides were relatively high.The best extraction process was as follows:liquid-to-material ratio was 35:1 mL/g,the microwave time was 19 min,and the microwave temperature was 94℃.The extracted crude polysaccharides of Chroogomphis rutilus were separated and purified through DEAE-52 and Sephaclyl S-400 chromatography columns to obtain a group of Botrytis cylindricus The polysaccharide fraction,and the polysaccharide fraction showed a single symmetrical peak,indicating that the separation and purification effect was better.(2)The results of the structural analysis of the separated and purified polysaccharides showed that the polysaccharide of Chroogomphis rutilus was composed of D-glucopyranose,mannose and galactose,mainly composed of 一6)-α-D-Glcp-(1→ And→4)-α-D-Glcp-(1→constitutes a triple helical heteropolysaccharide with a molecular weight of 135 kDa,a monosaccharide molar ratio of 1:0.02:0.03,and a regular network appearance under the scanning electron microscope;The atomic force electron microscope was spherical and irregular.(3)The results of in vitro and in vivo antioxidant activities of the isolated and purified Chroogomphis rutilus polysaccharides showed that the Chroogomphis rutilus polysaccharides had a certain total reducing power,·OH,ABTS+and ·O2-free radical scavenging ability,and it was in mice.In the antioxidant test,with the increasing dose of Chroogomphis rutilus polysaccharides,the weight,T-SOD activity,and total antioxidant capacity of the mice also increased,while the content of MDA gradually decreased,indicating that the Chroogomphis rutilus polysaccharides had certain antioxidant activity in vitro and in the vivo.(4)The in vivo immunomodulatory activity of the isolated and purified Chroogomphis rutilus polysaccharide showed that the polysaccharide of Chroogomphis rutilus polysaccharides had a significant alleviating effect on the decrease in immune function of mice induced by cyclophosphamide,and could significantly increase the weight,spleen and thymus of the mice index,peripheral blood white blood cell count,serum IgG,IgA,IgM content,macrophage phagocytic index,splenic lymphocyte proliferation ability,and could significantly increase the CD4+/CD8+value of splenic lymphocytes,improve liver damage caused by cyclophosphamide,and could Significantly increase the level of 3 cytokines in serum.It showed that the Chroogomphis rutilus polysaccharides could effectively relieve immunosuppression.