Protective Effect Of Scutellarin On Diabetic Cardiomyopathy

Diabetic cardiomyopathy（DCM）is one of the major cardiovascular complications that lead to the increase of mortality in diabetic patients.Its pathogenesis is complex.Continuous high glucose can cause inflammatory factor release,cell membrane function damage,myocardial cell apoptosis,ventricular dysfunction,and even lead to heart failure and lifethreatening.At present,there is no specific drug in clinical.Scutellarin is not only effective in the treatment of ischemic cardiovascular and cerebrovascular diseases,but also in the treatment of chronic complications of diabetes.In recent years,research shows that scutellarin has a certain effect on DCM.This paper studies the treatment of DCM by scutellarin,and probes into its mechanism,so as to provide scientific basis for its clinical application.Among the 96 c57BL/6J mice,16 mice were selected as the blank group random Ly,the other 80 mice were used as the experimental group and used to establish c57 diabetic mice model by injecting with Streptozocin（STZ）.The experimental group were divided into 5groups,including model group（DM）,low dose group（5 mg·kg-1）,middle dose group（10 mg·kg-1）,high dose group（20 mg·kg-1）and pioglitazone group（10 mg · kg-1）.Scutellarin on body weight and blood glucose of diabetic cardiomyopathy mice.By detecting the indexes of myocardial contractile function such as Fractional Shortening,FS,Ejection Fractions,EF,Left Ventricle end-diastolic,LVVd and Left Ventricle systolic volume,LVVs in mice.The serum biochemical indexes such as Creatine Kinase,MB Form,CKMB 、 Lactate dehydrogenase,LDH 、 cardiac troponin1,c Tn I 、 malondialdehyde,MDA、superoxide dismutase,SOD、catalase,CAT and glutathione peroxidase,GSHPx;The indexes of ELISA inflammatory factors such as interleukin-6,IL-6、interleukin-1β,IL-1β、interleukin-18,IL-18、Interferon-γ,IFN-γ、Tumor Necrosis Factor α,TNF-α and monocyte chemotactic protein 1,MCP-1.The Western blot method was used to detect the protein expression levels of IL-18 、Nucleotide-binding oligomerization domain-like receptor protein 3,NLRP3 、 transforming growth factor-β1,TGF-β1 、 IL-1β 、 nuclear factor erythroid 2-related factor 2,Nrf2、nuclear transcription factor kappa B,Nf-κB、protein kinase B and p-AKT.As a result,Scutellarin had no effect on the blood glucose of diabetic mice.It can significantly reduce the ratio of heart to body,increase EF,FS and LVVd,increase the activity of serum antioxidant enzymes,reduce the level of serum inflammatory factors,improve the contractile function of myocardium,and reduce the degree of pathological injury and fibrosis of myocardium.In conclusion,Scutellarin can significantly inhibit the degree of cardiac fibrosis in diabetic cardiomyopathy mice.It was found that Scutellarin had the effects of anti-oxidative stress and anti-inflammatory reaction.The molecular mechanism is closely related to the inhibition of NF-κB nuclear translocation,activation of NRf2 nuclear translocation and Akt phosphorylation.A total of 60 db/db mice were divided into 4 groups,including model group（DM）,DM + breviscapine low dose group（7.5 mg·kg-1）,DM + breviscapine medium dose group（15 mg·kg-1）,and DM + Breviscapine High Dose Group（30 mg·kg-1）.Thirty c57 mice were divided into two groups: Control group and Scutellarin（30 mg·kg-1）group.The activities of Creatine Kinase,CK and LDH in serum of mice were detected by biochemical indexes after 7 weeks administration.The contents of IL-1,IL-18 and Recombinant Human collagen-1,collagen1 were detected by ELISA.The results showed that Scutellarin had no effect on blood glucose and body weight of db/db mice,but it can reduce the myocardial tissue damage of diabetic cardiomyopathy mice.Scutellarin has obvious anti-inflammatory effect,and its mechanism needs to be further studied.