Identify And Analyze Micro RNA Editing And Mutations Potentially As Sociated With Alzheimer’s Disease Based On SmallRNA Sequencing Data

Animal microRNAs are a class of non-coding small molecules with a length of about 22 nt that participate in the regulation of gene expression.By complementary pairing with the untranslated region of the target gene,mi RNA can prevent the target gene from exerting its physiological function through cleavage degradation or translation inhibition.Brain tissue is rich in mi RNA,and some are unique to brain tissue,and play an important role in the development of the nervous system and the formation of synaptic plasticity.RNA editing means that RNA molecules are modified by some proteins during the generation process,so that nucleotide changes,deletions,or insertions occur.Nucleotide changes may cause the hairpin structure of the microRNA precursor to change,thereby changing the expression level of the mature microRNA;or the sequence of the mature microRNA,especially the seed region,may change to affect the microRNA recognition of the correct target gene.Alzheimer’s disease(AD)is a progressive neurological degenerative disease with a concealed onset and progressive development.The influence of microRNA expression in the brain due to RNA editing may be one of Alzheimer’s incentives.In this project,through high-throughput sequencing technology,that is,small RNA sequencing technology,in the NCBI,the brain sequencing data of 24 Alzheimer’s patients and the brain sequencing data of 86 normal humans were searched.A comprehensive analysis of these data was conducted using the Jsmall RNA bioinformatics integrated analysis toolkit developed by the laboratory.2450 editing events with significant editing level were identified,of which 56 A-to-I(G)events,24C-to-U events,and 57 SNP events.Of the 340 events,176 events were significantly reduced.Five microRNA-edited target genes were predicted,and it was found that there was an intersection with the three previously studied RNAs,and there is a high probability that they will be targets.These findings provide some basis for the subsequent study of the effects of RNA editing in Alzheimer’s disease.