Study On Inhibitory Effects And Mechanism Of Melittin On Human Prostate Cancer DU145 Cells

【Objective】In China,the incidence of prostate cancer has been increasing rapidly in recent years.For patients with advanced prostate cancer with local and systemic metastasis,the current treatment strategy will still bring heavy economic and psychological burden to patients.Therefore,to explore a new and practical treatment for prostate cancer is an important problem to be solved in the clinical work of urology.Biotoxins are widely used in traditional Chinese medicine,and a considerable part of them have significant antitumor effect.Among them,melittin(MEL),as a traditional anti-inflammatory and analgesic traditional Chinese medicine,has been proved to have potential antitumor effect by long-term clinical application.The purpose of this study was to investigate the antitumor activity of MEL on human prostate cancer cells in vitro and in vivo and its possible mechanism.【Methods】 The effects of MEL on the growth of DU145 cells were detected by MTT and plate cloning,and the morphological changes of DU145 cells after MEL treatment were observed under transmission electron microscope.Transwell experiment was used to analyze the changes of cell invasion ability of DU145 cells treated with MEL.Flow cytometry was used to analyze the cell cycle distribution of DU145 cells treated with MEL.Flow cytometry and TUNEL were used to observe the apoptosis of DU145 cells.Western blot was used to detect the expression of related proteins in DU145 cells after MEL treatment.Finally,the antitumor activity of MEL in vivo was evaluated in transplanted tumor model,and the expression of related proteins in tumor tissue was detected by immunohistochemistry.The potential role of MEL in regulating the growth of DU145 cells and the possible mechanism were analyzed.【Results】 MEL can significantly inhibit the proliferation and invasion of DU145 prostate cancer cells,induce cell cycle arrest of DU145 cells,and increase the proportion of apoptosis.The detection of apoptosis related protein expression further confirmed the above results.At the protein expression level,MEL significantly inhibited the phosphorylation of PI3 K,Akt and m TOR,suggesting that they play a key role in MEL-induced tumor cell death.In addition,MEL can significantly inhibit the growth of tumor in nude mice,and the results of immunohistochemistry in tumor tissue confirmed the above western blot results.【Conclusion】MEL could inhibit the proliferation of human prostate cancer DU145 cells by inducing cell cycle arrest and promote apoptosis.The mechanism may be related to the regulation of PI3 K / Akt / m TOR pathway accompanied by the regulation of related protein expression.