The Expression Of M1 And M2 Tumor-associated Macrophages In Hepatocellular Carcinoma And The Prognosis Of The Overall Survival Of Hepatic Carcinoma Patients And MiR-210-5p Mediated SPON2 Increases The Sensitivity Of Hepatic Carcinoma Cells To Sorafenib

Objective: To analyze the correlation between the proportion of M1 and M2 tumor-associated macrophages(TAM)in liver cancer tissue and the prognosis of liver cancer patients.To analyze the change of the TAM-M1 polarization gene SPON2 expression in hepatocellular carcinoma cells,knock down miR-210-5p in human hepatocellular carcinoma(HCC)cell lines to investigate whether miR-210-5p can cause hepatoma cells by regulating SPON2 Changes in sensitivity to sorafenib.Methods: The expression and prognostic correlation of TAM-M1 marker HLA-DR and TAM-M2 type marker RELM-β in normal tissues and cancer tissues of 68 liver cancer patients were detected by immunohistochemistry;real-time fluorescence quantitative PCR(QPCR)method to detect the expression of miR-210-5p and SPON2 in hepatoma cells;transfection of 100 n M miR-210-5p inhibitor and inhibitor NC in hepatoma cells lines SMMC-7721 and MHCC-97H;detection by CCK8 Changes in the sensitivity of hepatoma cells SMMC-7721 and MHCC-97 H to sorafenib after knocking down miR-210-5p.Results:(1)Immunohistochemistry results showed that the adjacent tissues and cancer tissues in 68 cases of liver cancer tissues were observed under a microscope 20 times.It was found that the median number of TAM-M1 counts in cancer tissues was 165,ranging from 0 to 964,the median counts in adjacent tissues was 334,ranging from 0 to 1003,the number of cells in TAM-M1 cancer tissues(165±23.43)is smaller than the adjacent tissues(333.32±36.43),the difference is statistically significant(p<0.05);the median number of TAM-M2 counts in cancer tissues is 487.8,range 0~1295,adjacent to cancer The median count in tissues was 299,ranging from 0 to 1033.The number of TAM-M2 cells in cancer tissues(487.76±53.84)was greater than the number of cells in adjacent tissues(299.02±40),the difference was statistically significant(p<0.05);(2)Compare the number of TAM-M1 and TAM-M2 in adjacent tissues with the number in cancer tissues,take the median(TAM-M1 marker HLA-DR: adjacent tissue/cancer tissue=1.32;TAM-M2 marker RELM-β: paracancerous tissue/cancerous tissue = 0.6)as a threshold,the patients were divided into a high-proportion group and a low-proportion group,and there was no difference in the survival between the two groups in patients with TAM-M1 positive expression Academic significance(p>0.05),the difference between the survival time(OS=21.5 vs 11 months)of the high proportion group and the low proportion group of patients with TAM-M2 positive expression was statistically significant(p<0.05);(3)q PCR results showed that the expression of SPON2 increased significantly after knocking down miR-210-5p in SMMC-7721 and MHCC-97H(p<0.05);(4)CCK8 test results showed that after knocking down miR-210-5p in SMMC-7721 and MHCC-97 H,it was found that Sorafenib increased the inhibition rate of liver cancer cells(IC50=18.23±2.1μM vs 25.44±3.26μM;17.69±3.74 μM vs 20.62±4.19 μM)(all p<0.05).Conclusion:(1)The distribution of TAM-M1 and TAM-M2 in liver cancer tissues is different between cancer tissues and adjacent tissues.There is no correlation between the distribution of TAM-M1 and the prognosis of liver cancer patients.The difference in the distribution of TAM-M2 has a significant correlation with the patient’s prognosis;(2)knocking down the miR-210-5p of hepatoma cells SMMC-7721 and MHCC-97 H can increase the expression level of SPON2;(3)knocking down the hepatoma cells SMMC-7721 and MHCC-97 H miR-210-5p can enhance the growth inhibition rate of sorafenib on Hepatocellular Carcinoma.