Chemical Compositions And Biological Activities Of Campylotropis Hirtella And Pharbitis Purpurea

This thesis is consisted of two chapters and summarized the research works during the master’s study.In the first chapter,the chemical constituents andβ-hematin formation inhibition activity of Campylotropis hirtella were dealt with.In the second chapter,the chemical constituents and biological activities of Pharbitis purpurea,were present.And the research progress of genus Pharbitis was summarized in the last part.Objective:To study the chemical compositions and biological activities of C.hirtella and P.purpurea.Methods:The compounds were isolated and purified by using various column chromatography methods including silica gel,Polyamide,Sephadex LH-20,MCI and preparative HPLC column chromatography.Their structures were identified based on spectral analysis.Furthermore theβ-hematin formation inhibition test and analgesic experiments were used to determine the activities of related compounds.Results:Twenty compounds were obtained from Et OAc-soluble part of 95%ethanol extract of underground parts of C.hirtella,and identified as hirtrllanone J（1）,isosophoranone（2）,myrsininone A（3）,3’-geranyl-5,7,4’,5’-tetrahydroxyisoflabone（4）,3（R）-2’-methoxyl-5,7,4’-trihydroxy-6-（3’’-methyl-2’’-butenyl）isoflavanone（5）,5,7,4’-trihydroxyisoflavone（6）,darbergioidin（7）,4’,5’-dihydroxy-5,7-dimethoxy-6-[3’’-methyl-2’’-butenyl]-coumestan（8）,hirtellanine B（9）,7,4’,5’-trihydroxy-5-meth-oxy-6-[3’’-methyl-2’’-butenyl]-coumestan（10）,isotrifoliol（11）,robipseudin A（12）,3（S）-2’,4’-dihyroxy-5’,5’-dimethoxy-（2’’,2’’-dimethylpyrano）-（5’’,6’’:7,6）isoflavan（13）,neorauflavane（14）,（+）-catechin（15）,（-）-gallocatechin（16）,β-sitosterol（17）,daucosterol（18）,Lupeol（19）,1,6-hexanolactam（20）.The compounds 1,2 and 12have showed betterβ-hematin formation inhibition activity and their half maximal inhibitory concentration were 26.7±0.5μg·m L^-1、29.5±0.4μg·m L^-1、31.8±1.4μg·m L^-1.Twelve compounds were isolated from Et OAc-soluble part of 75%Et OH-H₂O solution of the whole herb of P.purpurea,and were identified as friedelin（1）,β-friedelinol（2）,β-amyrin（3）,α-amyrin（4）,6β-hydroxy stigmast-4-en-3-one（5）,daucosterol（6）,β-sitosterol（7）,stigmasterol（8）,umbelliferone（9）,p-hydroxypheny-lethanol p-coumarate（10）,kaempferol-3-β-D-（6-O-cis-p-coumaroyl）glucopyranoside（11）,and glyceroylmonopalmitate（12）.The analgesic rates of compound 11,isolated from P.purpurea,was 55.70%in acetic acid writhing test in mice.Conclusion:It were flavonoids containing prentenyl/geranyl that isolated from C.hirtella and the structure containing prentenyl/geranyl group shows a betterβ-hematin formation inhibition activity.The compounds 1,2 and 12 which contain prentenyl/geranyl showed similar activities to the positive drug.The chemical constituents were mainly triterpenoids and phenylpropanoids of P.purpurea and several compounds had been reported with analgesic activities.Compound 11 showed a good activity in analgesic experiment.To some extent,it was proved that the plant has the rationality of analgesic activity.