Effects Of Baicalin On Proliferation And Apoptosis Of Gastric Cancer HGC-27 Cells

[Objective]To investigate the effects and mechanism of baicalein(BAI)on the proliferation and apoptosis of HGC-27 cells.[Methods]After HGC-27 cells were treated with different concentrations of BAI,the proliferation of cells was detected by MTT assay;the colony formation of cells was detected by plate colony formation;the lateral migration ability of cells was observed by scratch healing test;the longitudinal migration ability of cells was detected by Transwell cell migration test;apoptosis was observed by Hoechse33342 staining,and the apoptosis rate was detected by annexin V-FITC/PI double staining flow cytometry;The expression of EMT markers and apoptosis related proteins were detected by Western blot.[Results]1.MTT assay results showed that:compared with the control group,5,15 and 25μmol/L BAI treated gastric cancer HGC-27cells for 24h,48h and 72h respectively,cell proliferation was significantly inhibited(P<0.05 or P<0.01).2.The plate colony formation experiment showed that compared with the control group,5,15 and 25μmol/L BAI significantly inhibited the colony formaiton of HGC-27 cells(P<0.05 or P<0.01).3.The scratch healing experiment results showed that:compared with the control group,25μmol/L BAI significantly reduced the ability of lateral migration of HGC-27 cells in 24h and 48h(P<0.05).4.The results of transwell cell migration test showed that compared with the control group,the longitudinal migration ability of HGC-27 cells treated with 5,15 and 25μmol/L BAI for 48h decreased significantly(P<0.01).5.Hoechst33342 staining results showed that compared with the control group,the concentration of 5,15 and 25μmol/L BAI on HGC-27 cells showed bright blue apoptotic bodies,and the number of apoptotic bodies increased with the increase of BAI concentration.6.The results of flow cytometry detection showed that:compared with the control group,the apoptosis rate of HGC-27 cells in 5,15 and 25μmol/L BAI group was significantly higher.7.The results of Western blot showed that BAI significantly increased the expression of Cleaved Caspase-3 and decreased the expression of Bcl-2 and Bcl-2/Bax(P<0.05 or P<0.01),increased the expression of E-cadherin and decreased the expression of Vimentin(P<0.05 or P<0.01).[Conclusion]1.BAI can effectively inhibit the proliferation and induce apoptosis of HGC-27 cells,which is related to BAI regulating the expression level of pro-apoptotic protien Cleaved Caspase-3 and anti-apoptotic protiens Bcl-2 and Bcl-2/Bax.2.BAI can effectively inhibit the horizontal and vertical migration of HGC-27 cells,which is related to BAI regulating the expression levels of EMT-related proteins expression.