Correlational Analysis Of The SNPs And Primary Hepatic Cancer Prognosis In Yanbian Area

Background:PHC(Primary hepatic carcinoma)is a malignant tumor with high morbidity and mortality worldwide,generally referred to as liver cancer.Due to the characteristics of high malignancy,strong invasive ability and poor prognosis of primary liver cancer,its mortality rate has remained high,and because of the insignificant clinical manifestations,it is often misdiagnosed,missed the best treatment time,and lost the opportunity for surgery.Therefore,the monitoring and screening of high-risk groups,and efforts to achieve early detection,early diagnosis,and early treatment are the most effective ways to improve the prognosis of liver cancer patients and reduce mortality.The main examination methods of liver cancer include imaging examination,pathological examination and examination of liver cancer tumor markers.The main treatment methods are surgical resection,liver transplantation,transcatheter arterial chemoembolization,local ablation,radiotherapy,targeted therapy,traditional Chinese medicine and symptomatic treatment.Although there is a mature system for the diagnosis and treatment of primary liver cancer,we still encounter the problem of lack of basis for judging the prognosis effect in the selection of treatment methods in actual clinical treatment.In modern society,due to the maturity of technology at the nucleic acid(molecular)level and the completion of the Human Genome Project,people are paying more attention to exploring the relationship between tumors and genes and discovering the Gene polymorphism of single nucleotide polymorphism(SNP)can be called the basis for judging the prognosis of liver cancer patients.Objective:Analysis of the correlation between single nucleotide polymorphisms and the prognosis of patients with primary liver cancer in Yanbian area provides a theoretical basis for finding prognostic markers for patients with primary liver cancer.Materials and methods:1.Selection of SNP sites related to the prognosis of liver cancer patients:using TRANSFACR prediction software and Regulome-DB database to select single nucleotide polymorphism sites that may be related to liver cancer,and using NCBI-SNP database SNP loci with minor allele frequencie>10%in China.2.Patient selection and sample extraction:establish a systematic inclusion standard and collect a total of 369 primary liver cancer patients who received treatment at Yanbian Cancer Hospital and Yanbian University Affiliated Hospital from October 2010 to December 2018 and met the inclusion criteria example.Ask the patient to sign an informed consent form,record the patient’s various clinical data in detail,and collect fresh blood samples before the patient undergoes radiotherapy and chemotherapy.Use a whole blood nucleic acid extractor to extract DNA samples from the blood.Use a spectrophotometer to test the extracted DNA samples,and re-extract or delete the samples that do not meet the standard.3.Genotyping:design primers,use PCR-RFLP technology to perform digestion and electrophoresis on the extracted DNA samples,and determine the genotype of SNP sites by the length and quantity of the digested products.4.Statistical analysis:Use statistical software to analyze the relationship between various clinical data and the prognosis of liver cancer patients,and analyze the relationship between the genotype of SNP loci and the prognosis of liver cancer patients.Results:1.The rs970797 is related to the prognosis of PHC patients:By querying the database and Cox regression analysis,it was found that the secondary allele frequency of the rs970797 locus among the many loci in the Chinese population was 0.402 and the correlation with the prognosis of PHC patients was statistically significant.2.The relationship between various clinical characteristics and the prognosis of PHC patients:the analysis results of the relationship between the clinical and pathological characteristics of 369 patients with PHC and mortality and overall survival indicate whether the patient has a history of hepatitis and the type of hepatitis The daily alcohol intake,the clinicopathological stage of the tumor,and whether the patient received adjuvant therapy had a statistically significant effect on the mortality and overall survival time(log-rank P<0.05).,Gender,drinking status and smoking status had no statistically significant effect on mortality and overall survival time(log-rank P>0.05).3.Distribution of rs970797 genotypes in differentsubgroups:Chi-square test showed that.the three genotypes of rs970797(CC,CA,AA)at different age levels,ethnic groups,genders,drinking conditions,smoking status.There is no difference in the distribution of patients with different tumor pathological types and adjuvant treatment.4.The effect of rs970797 genotype on prognosis of patients:Kaplan-Meier survival analysis and Cox regression analysis showed that AA gene can be used as a protective factor for the prognosis of PHC patients,HR=0.709 in the recessive model of variant A allele,95%CI=0.508-0.991,P=0.044.Under the codominant model,HR=0.697.95%CI=0.486-1.001,P=0.05).5.Multivariate analysis:Multivariate Cox regression analysis showed that rs970797AA genotype,low tumor pathological stage and receiving adjuvant therapy were protective factors for the prognosis of PHC patients(HR<1,P<0.05).6.The situation of rs970797 minor allele frequency in people in different regions of the world:the minor allele frequency of rs970797 is 0.33,and the value found by querying the NCBI database is in comparison with other regions in the "Thousand Human Gene Project"The lower level is much lower than the global average of 0.42.7.Subgroup analysis afterwards:The patients were divided into subgroups according to age,gender,ethnicity,hepatitis,smoking,drinking,and adjuvant treatment,and then statistically found that:in these subgroups,patients with PHC carrying rs970797 AA gene and CC and CA genotypes The risk ratios of PHC patients were:0.671 in the male patient group(P<0.05),1.009 in the female patient group,and 0.618 in the Korean patient group(P<0.05),0.658(P<0.05)in patient group with history of hepatitis,0.849 in patient group without history of hepatitis,0.532(P<0.05)smoking group,0.592(P<0.05)in drinking group.Conclusion:1.Single nucleotide polymorphism rs970797(C/A)may be related to the overall survival of patients with PHC in Yanbian area.Patients with the rs970797A gene have a longer overall survival and a lower risk of death.2.Transcatheter arterial chemoembolization(TACE)can effectively prolong the overall survival of patients with PHC and reduce the risk of death.3.Daily alcohol intake may be related to the overall survival of patients with PHC in Yanbian.Patients with high daily alcohol intake have a shorter overall survival and a higher risk of death.