Anti-inflammatory And Antioxidant Biological Activities Of Cardamine Komarovii Flower Extracts

Cardamine komarovii flower is a cruciferous plant of genus Cardamine and is mainly distributed in China’s Heilongjiang Province,Jilin Province,Liaoning Province in Korean peninsula.Cardamine komarovii flowercan maintain hemostasis,reduce blood pressure,and induce sedation.This experiment takes Cardamine komarovii flower as the research object to study its anti-inflammatory effect and antioxidant biological activity,and it will provide a theoretical basis for the development and application of Cardamine komarovii flower.In this experiment,Cardamine komarovii flower extracts was used to detect the total flavonoid content by using aluminum nitrate colorimetry,and the high-resolution liquid chromatography was used to determine the flavonoid monomer composition.The results showed that the total flavonoid,myricetin,and quercetin contents of the Cardamine komarovii flower extracts are 41.13,2.88,and 0.36 mg/g,respectively.In vitro anti-inflammatory experiment,used different concentrations of Cardamine komarovii flower extracts to treat LPS-induced PMs.To prove its anti-inflammatory effect in vitro,we detected the levels of inflammatory mediators(NO,PGE2,TNF-α,IL-6 and IL-1β)and expression levels of related proteins in MAPK,NF-κB and JAK/STAT signaling in LPS-induced PMs by using ELISA,qPCR,immunoblotting and cell immunofluorescence.The results showed that the Cardamine komarovii flower extracts can inhibited the expression of NO,PGE2,TNF-α and IL-1β in vitro inflammation model with a dosage-dependent manner.It can inhibited the phosphorylation of P38 in MAPK signaling pathway,nuclear translocation of NF-κB and the phosphorylation of IRF3,STAT1,STAT3 in JAK/STAT in a dosage-dependent manner.It indicated that the Cardamine komarovii flower extracts can play an anti-inflammatory effect by regulating MAPK,NF-κB and JAK/STAT signaling pathways.In vitro antioxidant experiment,the DPPH free radical,ABTS free radical,iron ion chelating ability,ROS content and HO-1 antioxidant enzyme protein expression level were used to ascertain its antioxidant biological activity.The results showed that the Cardamine komarovii flower extracts had a good free radical scavenging ability in a dosage-dependent manner.It can inhibited the ROS produced by t-BHP-induced HepG2 cells.It can increased the expression of HO-1 antioxidant enzyme protein.In vivo anti-inflammatory experiment,LPS nasal drip mice were used to establish an acute lung injury model,and were divided into 5 groups,namely normal group,LPS group,Cardamine komarovii flower extracts low dose group(5 mg/kg),Cardamine komarovii flower extracts High-dose group(10 mg/kg),DEX(7.8 mg/kg).The normal group and the LPS group were given normal saline for 7 days,the Cardamine komarovii flower extracts low-dose group,and the Cardamine komarovii flower extracts high-dose group were given the corresponding concentration of extract for 7 days.The DEX group began intraperitoneal injection on the 6th and 7th days.Except for the normal group on the 8th day,all the other groups received intranasal LPS after anesthesia,and mice were lost after 24 hours,and samples were collected.We detected cytokine content、neutrophil infiltration,and lung tissue pathological damage in lung alveolar lavage fluid by using ELISA,flow cytometry and H&E.The results showed that the Cardamine komarovii flower extracts can reduced the protein concentration in the alveoli,the number of neutrophils in the alveolar lavage fluid and the expression of IL-1β and the lung injury score decreased significantly in a dosage-dependent manner.The results indicated that the Cardamine komarovii flower extracts can protect LPS-induced acute lung injury.