Drug Target Gene Prediction Of Staphylococcus Aureus

Objective: With the application of antibiotics and the change of ecological environment,the proportion of resistant Staphylococcus aureus is higher than that of wild or non resistant Staphylococcus aureus.The anti-bacterial weapons in patients and doctors’ hands are constantly upgrading.The perseverance of Staphylococcus aureus has led to the gradual reduction of all efforts.We still need to face the changing resistance of Staphylococcus aureus and its tenacious pathogenicity.In the modern era of antibiotics,since Sir Fleming,people have used penicillin,methoxycillin,vancomycin and other antibacterial drugs to treat the infection of Staphylococcus aureus.Staphylococcus aureus either produces β lactamase,or gets the gene fragment of antagonists from Enterococcus,or its own gene begins to adapt to the mutation of antibiotic environment.The war between Staphylococcus aureus and human antibiotics has never stopped.Therefore,it is necessary to search for the source and mechanism of drug resistance of Staphylococcus aureus from the gene level,to summarize the drug target of resistant strains of Staphylococcus aureus,to master the dynamic changes of drug resistance genes,to explore the clinical significance of drug resistance gene changes and to find new antibiotics.High expression genes,essential genes,genome island genes and species specific genes are more likely to be effective drug targets in bacteria.In recent years,bioinformatics has advantages in cost,time and automation in drug target gene screening.We can use bioinformatics to screen drug target gene of Staphylococcus aureus from gene level.Methods: The genome and annotation information of 49 strains of Staphylococcus aureus were downloaded from NCBI database,and 2767 bacterial genomes were obtained for re annotation.In combination with the re annotation software such as Zcurve,Prodigal,Glimmer the genome of S.aureus was re annotated,61 genes were predicted to be missing,and new ORFs were identified.The new genes were annotated by homology comparison.The protein expression data of 49 strains of Staphylococcus aureus were downloaded from paxdb,and 10% of the highest expression genes were selected as potential high expression genes.The essential genes of 49 strains of Staphylococcus aureus were selected after downloading from the required gene database,and then were gradually extended to 49 strains of Staphylococcus aureus after the intersection of S.aureus N315 and S.aureus NCTC.170 essential genes were obtained after repeated intersection.Obtain the sequenced genome island genes from the website of islandviewer.And 32 genomic island genes of Staphylococcus aureus were identified by selecting the genomic island sequences and comparing with the homologous blast of NCBI.The genes of human and beneficial intestinal flora were excluded by homology comparison.Finally,the action pathway protein of the target drug and whether the drug can be made were searched and verified on the Dragbank.Results: Through repeated screening,10 genes such as SAZ172＿0418,SAZ172＿0420,SAZ172＿1976,SAZ172＿1978,SAZ172＿1980,SACOL1524,SA2981＿1507,SA2981＿2326,SA2981＿2521,SA2981＿1955 may be the drug target genes after eliminating the beneficial intestinal flora.Conclusion: 1.The research method in this paper can be used as one of the methods for the development or renewal of antimicrobial agents of various microorganisms.2.The 10 genes selected from this method provide theoretical will help for the research and development of broad-spectrum antibiotics of Staphylococcus aureus resistant strains.3.The corresponding drugs of 10 potential drug target genes will not lead to the occurrence of various complications and side effects on human body.