A Preliminary Study On LncRNA RP1-90L14.1 Function And Regulation Mechanism In Prostate Cancer

Objective:To investigate the expression level,biological function and mechanism of lnc RNA RP1-90L14.1 in prostate cancer cells.Methods: The RT-PCR was used to detect m RNA expression levels of two different prostate cancer cell lines,LNCa P and LNCa P-AI.The RP1-90L14.1 overexpression plasmid and the negative control plasmid were transiently transfected into LNCa P cells with low level of RP1-90L14.1,called LNCa P-RP1-90L14.1 group and LNCa P-NC group.The LNCa P-RP1-90L14.1 group and the LNCa P-NC group were simultaneously cultured in a common medium and a carbon-adsorbed phenol-free red medium.Then,the proliferation,migration activities,and invasion activities were measured by CCK-8 assay,transwell migration test,and transwell invasion test,respectively.Last,m RNA and protein expression levels of the LNCa P-RP1-90L14.1 group and the LNCa P-NC group cultured in a common medium were measured by RT-PCR and Western Blot for GRIN2 A.The m RNA expression levels of the LNCa P-RP1-90L14.1 group and the LNCa P-NC group cultured in a common medium were measured by RT-PCR for mi RNA-142-3p.Results:1.The expression level of RP1-90L14.1 in LNCa P-AI cells was higher than that of LNCa P cells(8.49±0.43 vs 2.53±0.95,P <0.05).2.After transfection with RP1-90L14.1,the expression of RP1-90L14.1 in LNCa P-RP1-90L14.1 group was significantly higher than that in LNCa P-NC group(0.71±0.22 vs 0.02±0.01,P <0.05).3.In the normal medium and serum-free activated carbon adsorption medium,the OD value of LNCa P-RP1-90L14.1 group was higher than the LNCa P-NC group,51.95%(1.22±0.08 vs 0.08±0.05,P <0.05)and 50.69%(0.79±0.02 vs 0.53±0.05,P <0.05)(72h),51.72%(1.72±0.07 vs 1.13±0.05,P <0.05)and 60.23%(1.18 ± 0.05 vs 0.73 ± 0.08,P <0.05)(96h).4.The migration abilities of the LNCa P-RP1-90L14.1 group was higher in both two mediums [(682.0±42.7)vs(422.0±37.1),(419.0±42.9)vs(251.0±25.9),P <0.05];the invasive ability of the LNCa P-RP1-90L14.1 group was significantly higher in both two mediums[(507.0±22.2)vs(274.0±19.6),(352.0±14.1)vs(216.0±14.3),P <0.05].5.After up-regulating the level of RP1-90L14.1 in LNCa P cells,the gene expression of GRIN2 A was increased[(5.13±0.89)vs(2.09±0.54),(5.88±0.29)vs(2.03±0.22),P<0.05].6.After up-regulating the level of RP1-90L14.1 in LNCa P cells,the gene expression of mi RNA-142-3p was inhibited(1.58±0.16 vs 2.55±0.42,P <0.05).Conclusion: 1.lnc RNA RP1-90L14.1 plays an important role in regulating the proliferation,migration and invasion of prostate cancer cells.2.RP1-90L14.1 may regulate the expression of GRIN2 A,mi RNA-142-3p as an endogenous competitive RNA,and promote the progression of prostate cancer.