Preparation And In Vitro Study Of Antibacterial Biomembranes For Periodontitis Therapy

Objective:The aim of this study was to prepare and study the properties of the antibacterial membranes using methoxy poly(ethylene glycol)-poly(ε-caprolactone-co-l-lactide)(mPEG-PCLA)as membrane material,with the different proportion of metronidazole,ornidazole and minocycline hydrochloride,respectively,in order to develop a new drug membrane with good antibacterial effect for the periodontitis therapyMethods:The drug-loaded membranes were prepared by solvent casting method with the proportion of 5 wt%,8 wt%,and 10 wt%,respectively.In vitro drug release properties were determined by ultraviolet spectrophotometric method.The antibacterial activities against Streptococcus mutans,Fusobacterium nucleatum and Porphyromonas gingivalis in vitro were observed on solid culture medium,and the antimicrobial effects on biofilms were studied by confocal laser scanning microscopy.The cytotoxicity of the MH membranes was evaluated by the extract liquid taken in the cell culture of the specimensResults:The membranes had the high drug loadings and slow-release performance.Drug release time was shortened with the increase in the content of drugs,the metronidazole and ornidazole membranes can achieve 10 days,and minocycline hydrochloride drug membranes can reach more than two weeks.Metronidazole and ornidazole drug membranes had better inhibitory effect on F.nucleatum than S.mutans.While minocycline hydrochloride membrane had a strong inhibitory effect on P.gingivalis and F.nucleatum,including S.mutans.As drug content increased,the antibacterial activities also increasedConclusions:In the study,we successfully prepared the metronidazole,ornidazole and minocycline hydrochloride drug-loaded membranes,and we found that the 10wt%minocycline hydrochloride drug-loaded membrane is expected to be used for the treatment of periodontal disease because of the obvious effect of periodontal pathogens inhibition and good sustained-release performance as well as no adverse effect on the growth of human periodontal ligament fibroblast.