An Fundamental Study: The Effects Of NHA-GO/GO And MiR-424 On Mesenchymal Stem Cell For Osteogenic Differentiation

ObjectivesIn this study,we will investigate the effects of nHA-GO/GO on proliferation and osteogenic differentiation of h ASCs and the role of mi R-424 in regulating h ASCs osteogenesis,which may provide a new insight for bone defect repair in bone tissue engineering.Materials and Methods1.h ASCs were seeded on nHA-GO/GO scaffolds for a systematic evaluation of cell adhesion,proliferation and osteogenic differentiation;the efficacy of the composite of h ASCs and scaffolds on new bone formation in nude mouses was investigated.2.Construction of lentiviral vectors encoding mi R-424 or inhibitor.After h ASCs were tracsfected by lentiviral vectors,quantitative real time PCR(q PCR),western blot,alkaline phosphatse and alizarin red staining were performed to determine the effecs of mi R-424 on osteogenic differentiation of h ASCs.Results1.The nHA-GO scaffold didn’t suppress cell adhesion and proliferation,and promoted the osteogenic differentiation of h ASCs.In vivo assays showed that nHA-GO scaffold improved the new bone formation in nude mouses.2.The overexpression of mi R-424 inhibitored h ASCs osteogenesis,while knockdown of mi R-424 promoted h ASCs osteogenesis and Smad5 may be the target gene of mi R-424.Conculsions1.The nHA-GO scaffold didn’t inhibitor h ASCs proliferation and promoted osteogenic differentiation in vitro and further increased new bone formation in vivo,suggesting that the nHA-GO scaffold holds great potential in bone tissue regeneration.2.The overexpression of mi R-424 inhibitored h ASCs osteogenesis,and there is a new potential target to regulate the osteogenic differentiation of h ASCs.