Ferritin Heavy Chain 1 Carried By Dendrimer Nanoparticles Vector Transfecting Natural Killer 92 Cells And MR Imaging In Vitro And In Vivo

Objective:The PEGylated dendrimer-encapsulated nanogold particles were used as a non-viral vector to transfer human ferritin heavy chain(FTH1)into natural killer cells 92(NK-92 cells)and subjected to MRI in vitro and in vivo.Methods:The fifth-generation polyamidoamine dendrimer was modified with polyethylene glycol(PEG)and encapsulated with nanogold particles as a non-viral vector to transfect the FTH1 gene into NK-92 cells.The synthesized support materials were characterized by magnetic resonance spectroscopy,UV spectrophotometry and transmission electron microscopy.The characteristics of the detectors were determined by agarose gel electrophoresis,CCK-8 kit,and hydrodynamic particle size.The fluorescence microscope was used.The transfection efficiency was measured by flow cytometry;NK-92 cells transfected with FTH1 were imaged in vivo and in vitro using 3.0 T MRI.Results:The synthesized pegylated dendrimer {(Au0)25-G5.NH2-mPEG17} DENPs with encapsulated nanogold particles has good biocompatibility,and the material is less cytotoxic,with a nitrogen to phosphorous ratio of 1 The transfection efficiencies of 1:1,2:1,5:1,and 10:1 were 57.8%,64.0%,80.2%,and 38.3%,respectively.Under the conditions of 200 and 500μmol/L ferric ammonium citrate,MRI examination revealed that T2-weighted images were all decreased,and the latter was more pronounced.T2-weighted images of tumor-bearing mice also showed corresponding changes.Conclusion:{(Au0)25-G5.NH2-mPEG17}DENPs can successfully transfect target genes.MRI can effectively detect NK-92 cells after FTH1 transfection in vivo and in vitro,which provides further clinical application of MRI visualization to evaluate the clinical efficacy of NK cell adoptive immunotherapy.