| Background:Kidney ischemia-reperfusion injury is refers to the kidney damage during ischemia and then restore perfusion on,It can happened in the renal transplantation,nephron sparing surgery diseases such as severe trauma,uncontrolled hemorrhagi,c shock in common.Renal IRI can not only cause acute renal injury(AKI),but also be an important cause of chronic renal disease(CKD),and ultimately lead to end-stage renal failure.Currently,there is no ideal method to treat renal IRI in clinic.Adipose stromal vascular fraction(ad-SVF)is a new stem cell source containing MSC and endothelial progenitor cells isolated from adipose tissue.Studies have shown that ad-SVF can reduce renal tubular injury and improve renal function.But the mechanism of ad-SVF repair kidney injury is unclear,the source of the outside body secretion in the tissue damage repair shows a good application potential.We studied renal function and renal pathological changes by establishing rat renal IRI model and ad-svf-exosome-infusion transplantation.To discuss the prevention and treatment of renal IRI in rats with ad-SVF-exosomes.Objective:To study the effect of AD-SVF-derived exosomes on renal IRI and explore the mechanism of AD-SVF-derived exosomes against AKI of IRI repair in rats.Methods:Forty eight SD rats after resection,right kidney to establish animal model of isolated kidney of the rats with random method above can be divided into four groups,namely the control group(Sham),renal ischemia reperfusion injury(IRI),ad-SVF group(SVF)and AD-SVF derived exosomes(group Exos).Inl、3、7d after reperfusion,blood sample was collected for detected serum creatinine(SCr)and blood urea nitrogen(BUN).In addition,kidney tissues were retrieved for hematoxylin and eosin(H&E)staining and immunohistochemical staining.Kidney injury score as well as characteristics of cells proliferation,apotosis and angiogenesis of peri-tubular were also evaluated.Results:Exosomes were observed under electron microscope,and the diameter of ad-SVF derived exosomes was mainly 40-100nm.ad-SVF derived exosomes could express CD9 and CD63 signaling proteins by Western blot.After 1,3,7d of the surgery of IRI,The Scr of group Exos and group SVF were significantly lower than group IRI(P<0.05).HE staining indicated that injury score in Exos group and SVF group were significantly lower than that in IRI group at 1、3、7d(1.9±0.2or1.8±0.5 vs.2.6±0.5;3.9±0.6 or 2.0±0.3 vs.3.2±0.4,and 2.4±0.6 or2.1±0.5 vs.3.9±0.6,P<0.05).The numbers of proliferative cells positive for Ki67 in Exos group and SVF group were significantly higher than that in IRI group at 3d(86.7±6.5 or 92.2±5.3 vs.27.0±5.8,P<0.05).Percentage of E-cadherin positive cells in Exos group and SVF group were significantly higher than that in IRI group at 3d(9.8±4.9 or10.5±4.3 vs.6.3±2.7,P<0.05).In addition,peritubular micro vascular numbers of Exos group and SVF group were 255.8±30.4 and 215.7±21.5,which were significantly higher than 98.6±15.2 In IRI group(P<0.05).Conclusion:ad-svf-derived exosomes can accelerate the restoration of damaged kidney after AKI of IRI in rats,providing a new strategy for renoprotection of renal ischemia reperfusion injury. |
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