Study On The Chemical Constituents And Anti-tumor Activity Of Veronica Sibirica

In this study,twenty-nine compounds were obtained by means of various isolation and purification technology such as HPLC,silica gel column chromatography,Sephadex LH-20 column chromatography and recrystallization from petroleum ether、ethyl acetate and n-butanol fractions of rhizome of Veronica sibirica,and they were identified as tanshinone Ⅱ A(1),tanshinone Ⅰ(2),dihydrotanshinone Ⅰ(3),cryptotanshinone(4),steraic acid(5),n-teracosanoic acid(6),β-sitosterol(7),octacosanoic acid octadecanyl ester(8),vanillic acid(9),isoferulic acid(10),3,4-dimethoxy cinnami acid(11),ferulic acid(12),daucosterol(13),protocatechuic acid(14),minecoside(15),chlorogenic acid(16),D-mannitol(17),geinposide(18),geinposidic acid(19),picrosid Ⅰ(20),picroside Ⅱ(21),6-O-veratroylcatalposide(22),6-O-(3"-O-trans-p-coumaroyl)-α-L-rhamnopyranosylcatalpol(23),2",3"-dicinnamoyl6-O-rhamnopyranosylcatalpol(24),3",4"-dicinnamoyl-6-O-rhamnopyranosylcatalpol(25),catalpol(26),rosmarinic acid(27),plantamajoside(28)and plantainoside D(29)on the basis of spectroscopic(1H-NMR,13C-NMR,MS,ect)and physicochemical methods.Among them,compounds 6,8,9,14,16,18,19,20,21,23,24,25,27,28,29 were isolated from the plant for the first time.The isolated compounds were evaluated for their inhibitory effects on HIF-la activation in Hep3B cells to estimate anti-tumor activity in vitro,and cell viability was measured by MTT assay.As a result,compounds 2、12、14、15、16、18-21、23-29 exhibited significant HIF-1α inhibitory activities.All compounds did not show obvious cytotoxicity.