The Study On Establishment Of Rat Models With Salpingitis Infertility

Objective:Fenestration of Staphylococcus aureus,Escherichia coli directly inoculated rat fallopian tube,the successful establishment of the fallopian tube inflammation infertility model of bacteria concentration,fallopian tube inflammation acute and chronic phase change and the body fluid metabolism of the discharge time,and to Gexia Zhuyu Decoction addition and subtraction intervention,observe the blood flow And the influencing factors of TNF-α and IFN-γ on hemodynamics and tubal tissue,and to explore the occurrence and treatment.Methods:The rat model of hemorrhagic infertility was established with 6×106/ml Escherichia coli and 4×109/ml Staphylococcus aureus.On the 7th,14th,21st and 28th day of the model,the culture of tissue culture Detection and observation of the pregnancy rate;Histopathological examination,histopathological examination and pregnancy rate were observed.2.Staphylococcus aureus to establish model modeling Day 3 began every day to take the pathology detection and tissue culture.3.Staphylococcus aureus model was established by subtractive subtractive method.The hemorrheology and TNF-α and IFN-γ immunohistochemistry were observed and analyzed statistically.Results:1.Under the light microscope to observe the 28th day of the fallopian tube for the chronic typical period;On the 17th day,the fallopian tube was transformed from acute to chronic salpingitis.3.The first 23 days of Staphylococcus aureus completely metabolized from the body.4.Gexia Zhuyu Decoction addition group and Kang Fu Yan group hemorheology indicators have improved to varying degrees,Gexia Zhuyu Decoction addition improved significantly.The expression of TNF-α and IFN-γ in the tissue was significantly decreased by Gexia Zhuyu Decoction addition.Conclusion:1.6 × 106/ml Escherichia coli,4×109/ml Staphylococcus aureus can be successfully copied rat oviductitis infertility model.2.Fallopian tube inflammation infertility is in the chronic aseptic environment to stimulate the formation of inflammation.3.Gexia Zhuyu Decoction addition can significantly improve the blood viscosity of the model of tubal inflammation infertility.4.Gexia Zhuyu Decoction addition can effectively inhibit the model of rat fallopian tube immune response damage,inflammatory stimulation.