The Research On The Correlation Between Myeloproliferative Neoplasms Patients JAK2V617F Gene And The Clinical Result Of Blood Cells Separation And Collection Cure Method

Objectives:Analyze the correlation between JAK2V617F mutation and the collection cure method of the separation and collection of blood cells applied on the patients suffering PV and ET.Try to provide highly effective and well-aimed collection methods and to find the best collection cure time,in order to rapidly reduce the count of peripheral blood cells,to well prevent the complications related to the disease,to shorten the drugtaken period and reduce the drug-taken dosage,to mitigate or avoid the side effect from the drugs,to provide reference to clinical care and to provide choices for individualized cure methods.Methods:Select 294 patients(male:186,female:108,average age:61)who had been diagnosed as BCR/ABL negative fusion gene PV and ET,and had been hospitalized in The First Affiliated Hospital of Dalian Medical University from February 2012 to December 2015.Among them,144 PV patients(male:90,female:54,average age:58)had been treated with TAE method;150 ET patients(male:96,female:54,average age:65)had been treated with TPR method.All the patients had only been treated with blood cells separation and collection method once.1.Analyze the JAK2V617F mutation of 294 patients through real-time fluorescence quantitative PCR method.2.Use MCS+enhanced multifunctional blood cells separation machine to treat 144 PV patients and 150 ET patients with TAE and TRP cure methods respectively.3.Analyze the blood cells count of 294 patients before collection,one hour after collection,one day after collection and one week after collection.4.Analyze the correlation between JAK2V617F mutation and the collection cure method of the separation and collection of blood cells applied on the patients suffering PV and ET.Results:1.The total positive mutation rate of JAK2V617F gene among BCR/AL negative fusion gene classical myeloproliferative disorder tumor patients is 73.47%.The rate of PV patients and ET patients are 79.71%and 68.00%respectively.2.The HCT and HB count of JAK2V617F positive mutation group is higher than JAK2V617F negative mutation group among PV patients(P<0.05).The WBC count of JAK2V617F positive mutation group is higher than JAK2V617F negative mutation group among ET patients(P<0.05).3.The rate of positive mutation increase with the rise of the count of HCT,HB and RBC among JAK2V617F positive mutation group of PV patients(P<0.05),the rate of positive mutation increase with the rise of the count of WBC among ET patients(P<0.05).4.When the two series of blood cells count increase simultaneously,more JAK2V617F positive mutation group cases are detected than that of negative group(P<0.05).When three series of blood cells count increase simultaneously,the rate of JAK2V617 mutation could reach 100%and all manifested as positive mutation.5.Among PV patients under TAE treatment,the HCT and HB count wiping result is more significant within JAK2V617 positive mutation group than that within negative mutation group(P<0.05).Among ET patients under TPR treatment,PLT count manifest statistic difference between JAK2V617F positive mutation group and negative mutation group(P<0.05).6.On the subject of end-product and spillage,JAK2V617F positive mutation group enjoyed a more significant statistic meaning than that of negative mutation group(P<0.05).Conclusions:1.JAK2V617F gene is the important molecular pathogenesis for PV and ET patients.JAK2V617F positive mutation cause the abnormal increase of peripheral blood cells.2.The HCT and HB count of JAK2V617F positive mutation group is higher than JAK2V617F negative mutation group among PV patients.The WBC count of JAK2V617F positive mutation group is higher than JAK2V617F negative mutation group among ET patients.3.The result of TAE treatment shows that the HCT and HB count collection result is more significant within JAK2V617 positive mutation group than that within negative mutation group.The result of TPR treatment shows that PLT,WBC and D-Dimer count wiping result is more significant within JAK2V617 positive mutation group than that within negative mutation group.JAK2V617F mutation may have certain effect on blood cells separation cure result.4.JAK2V617F positive mutation group enjoys more significant collection result which shortened the drug-taken period and reduced the drug-taken dosage,mitigated or prevented the side effect from the drugs.5.Improve the collection cure result for JAK2V617F negative mutation patients by increasing the quantity of treated blood and the times of treatment,combined with timely drugs prescription and the optimized the collection methods like discontinuous and repetitive blood cells separation.