Stady On Preparation And Quality Standards Of Floating Tablets In Stomach Of The Extraction From Rhizoma Menispermi

[Object]Extraction from Rhizoma Menispermi of this article as the stomach drug floating tables explore hydroxypropyl methylcellulose as hydrophilic matrix material,MCC as filler,Dauricine a total base of active ingredient stomach Preparation of internal floating chip investigated in vitro release and the film has been studied quality standards;preliminary toxicity North bean root extract extract acute toxicity study in toxicology;MTT assay extraction from Rhizoma Menispermi inhibition of gastric cancer cells SGC7901 prove Menispermi extract toxic substances and have anti-cancer activity in vitro;above experiment Menispermi extract made from gastric floating tablets provide scientific basis.[Method]1.Extraction from Rhizoma Menispermi is acidic water percolation method;spectrophotometry and by HPLC respectively Menispermi extract Dauricine and dauricine total alkali content measurement and methodology inspection.Determination LD50.North bean root extract 2.The use of modified Karber method.3.By MTT assay concentration gradient through a number of negative and positive control group and the intervention group administration,which are affected to varying degrees on SGC7910 were measured inhibition rate trends 4.Preparation of this article focuses on the complex floating pieces to act as tablets and bleaching time,hold time drift,tablets and other aesthetic level as indexes of the materials were single factor;cumulative release of the drug in order to investigate the factors comprehensive Review Q as index by L9(34)orthogonal design process optimization,to determine the best preparation.5.Establish Menispermi extract complex floating sheet quality standards,tablets qualitative and quantitative performance and floating routine examination.[Results]1.Extraction from Rhizoma Menispermi using acid extraction alkali Shen alcohol reflux method in the system,measuring medium 0.5mol/L hydrochloric acid and pure water volume ratio of 1:450,detection wavelength of 280nm,by method validation,Total Base in 0.018～0.048mg·mL-1 concentration a good linear relationship(r2=0.9987),precision,stability,RSD values of repeated experiment were less than 2%,the recovery rate is greater than 99%,to meet the requirements.2.Adoption of the preliminary experiment to determine the value Dn and Dm dose North bean root extract were 3000 mg·kg-1,1300 mg·kg-1,the use of statistical methods,LD50=1940.3 mg·kg-1,95%CI 1742.89～2149.89 mg · kg-1.3.After 48 and 72 hours,withl μg/mL、5μg/mL、25μg/m、50μg/mL、100μg/mL of extraction from Rhizoma Menispermi,All of SGC7901 gastric cancer cells showed a significant inhibitory effect showed time and concentration-dependent manner,with respect to 100μg·mL-1 5-FU positive control group showed a more significant inhibition 4.determine the best prescription:main drug 92.80mg;HPMC 50mg;CMS-Na 30mg;NaHCO3 65mg;octadecanol 20mg;MCC 135mg,released by curve fitting verification:zero-order kinetics and Higuchi equation could better describe North bean root extract gastric floating tablets in gastric juice in vitro release curve.5.thin layer chromatography,high temperature,humidity,light,buoyancy,equilibrium solubility,partition coefficient properties were identified.[Conclusion]Extraction from Rhizoma Menispermi complex floating piece look smooth and extended the advantage in the stomach residence time and sustained release,to meet the design requirements,improve the treatment of gastric cancer,and traditional Chinese medicine complex floating sustained release Study sheet accumulated some experience and data.