| Objective To investigate the pathophysiogical mechanism of oxidation stress-induced CCH in elderly rats and how the electric acupuncture work on it.Methods In experiment one,30 adult cleansing-grade experimental Sprague-Dawley male rats were divided into two groups:adult shame surgery group(group YS)and adult model group(group YM)while in experiment two,75 nature-aged Sprague-Dawley male rats were divided into 5 groups:old shame group(group OS),old model group(group OM),Candesartan treatment group(group CA),Perindopril treatment group(group PE)and the electric acupuncture treatment group(group EA).The CCH model was established by 2-VO(bilateral common carotid artery occlusion)undergoing isoflurane inhalational anesthesia.Different groups were received different treatments for the first time 2 hours after the surgery:Group EA received electric acupuncture therapy at the acupoints of "Baihui" and "Dazhui",group CA and group PE received intragastric administration,and the other groups received binding control or/and intragastric administration to make a balance.All the rats would be tested respectively with blood pressure,Cerebral blood flow(CBF),Morris water maze,open field test and new object recognition before they were executed to death.Observation indicators:(1)Detected cognitive function by Morris water maze test,open field test and novel object recognition test.(2)Measured the levels of ROS in blood serum,the AngⅡ,NOX,ROS,MDA,SOD,H2O2,CAT and GSH-Px in hippocampus by enzyme linked immunoadsorbent assay(ELISA).Results Experiment One The cerebral blood flow observation shows:at the same time compare the surgery group’s rate of CBF change with the shame surgery group’s,the surgery group’s rate of CBF change were significantly decreased(P<0.01).Compared YS with the OS at the same time,numerical values the trend were similar(P>0.05).Compared YM with the OM at the same time,the trends were similar(P>0.05),but in 6 weak OM’s rate of CBF change was significantly decreased(P<0.01).The water maze test shows:1)the number to cross the original platform during space exploration:numerical values were similar analyzed by reperted measurement experiment(P>0.05)and the distance to the platform of each group was shorter and shorter.Compared to YS,OS’s swimming distance was significantly reduced(P<0.01).Compared with OS,OM’s swimming distance was reduced and there was a significant difference in last three days(P<0.01).Compared with YS,OS’s swimming distance was longer,but there was statistically significant from the third to fifth days(P<0.05).Compared to YM,OM’s swimming distance was longer,but there was no statistically significant difference(P>0.05).2)escape latency shows:compared with shame surgery group,surgery group’s escape latency were lower(P<0.05,P<0.01).Compared with OS,OM’s escape latency was reduced(P<0.05).Compared to YM,OM’s escape latency was significantly reduced(P<0.01).The open-field test shows:after surgery,the ratio for resting time of inside and outside zone was significantly reduced(P<0.01).Compared with YS,OS’s ratio was lower(P<0.05).Compared with YM,OM’s ratio was significantly reduced(P<0.01).The new object recognition test shows:compared with the shame surgery group,model group’s time of new object recognition was decreased significantly(P<0.01).Compared to OS,OM’s time of new object recognition was decreased,but there was no statistically significant difference(P>0.05).Compared to YM,OM’s time of new object recognition was significantly decreased(P<0.01).ELISA shows:compared with shame surgery group,model group’s hippocampal concentration of AngⅡ,NOX,ROS,SOD and MDA were significantly higher(P<0.01,P<0.05).Compared to OS;YS’s hippocampal concentration of AngⅡ,NOX,ROS,SOD and MDA was increased,but only SOD was statistically difference(P<0.05).Compared to OS,OM’s hippocampal concentration of AngⅡ,NOX,ROS,SOD and MDA concentration was increased,but only AngⅡ,NOX statistically significant(P<0.05).Experiment Two The cerebral blood flow observation shows:every Groups’ CBF were restored,but compared with shame surgey group,the other group is still significantly lower compared with the same time(P<0.01).Compared with model group,treatment group was no statistical difference on the first day afert surgery(P>0.05),and the treatment group’s CBF restore faster after 1 week(P<0.01,P<0.05),but there were no statistical difference between treatment group in 6 weeks(P>0.05).The water maze test 1)the number to cross the original platform during space exploration:numerical values were similar analyzed by reperted measurement experiment(P>0.05)and the distance to the platform of each group was shorter and shorter.Compared with OS at the same time,the model group’s first day to fifth day,CA’s third and fourth days,and PE’s third and the fourth day,the third day of EA were statistically significant(P<0.01,P<0.05).Compared with OM group at the same time,the first day of CA,the third and fourth day of PE,the fifth day of EA were statistically significant(P<0.01,P<0.05).2)escape latency shows:compared with OS,each group’s escape latency is shorter,OM and PE were statistically significant(P<0.01,P<0.05),while CA and EA had no statistical significance(P>0.05).Compared with OM,the treatment group were significantly increased(P<0.01).But there was no statistical difference between the treatment group(P>0.05).The open-field test shows:after surgery,the ratio for resting time of inside and outside zone was significantly reduced(P<0.01).After treatment,the ratio were significantly increased(P<0.01).But there was no statistical difference between treatment groups(P>0.05).The new object recognition test shows:compared with the shame surgery group,model group’s time of new object recognition was decreased significantly(P<0.01).Compared with OM,treatment group recognition time significantly increased(P<0.01).But there was no statistically significant difference between the treatment group(P>0.05).ELISA shows:Compared with OS,OM and treatment’s hippocampal concentration of AngⅡ,NOX,ROS,MDA,H2O2 were significantly higher(P<0.05,P<0.01)while the concentration of SOD,CAT and GSH-Px were decreased(P<0.05,P<0.01).Compared with OM,the treatment group hippocampal concentration of AngⅡ,NOX,ROS,MDA,H2O2 concentration were significantly lower(P<0.05,P<0.01),while the concentration of SOD,CAT and GSH-Px were increased(P<0.05,P<0.01).But there was no statistical difference between treatment groups(P>0.05).Conclusions(1)The rises of the level of oxidative stress is one of risk factors of cognitive impairment in aged CCH rats.(2)The activation state of AngⅡ-NOX-ROS in aged CCH rats is one of the mechanism of nerve injury of hippocampus.(3)By regulating the expression of AngⅡ and NOX,reducing the release of ROS,electric acupuncture play an important role in neural protection under CCH. |
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