| Background:Special AT-rich sequence-binding protein(SATB1)associate with the growth,differentiation and maturity of T cells and involve in the tomour growth and metastasis by regulating the expression of related genes through its specific structure,its role has been confirmed in several tumor forms.Objective: To study the relationship between SATB1 expression and liver cancer metastasis,and Investigate its clinical pathological significance in the diagnosis of the liver cancer.Methods:1.We examined the SATB1 m RNA and protein expression by immunohistochemistry,PCR and western blot in hepatocellular carcinoma tissue and two kinds of cancer cell lines,MHCC-97H(high metastatic potential)and Hep G2(low metastatic potential)respectively.2.Silencing plasmids and overexpression plasmid were used to silence the SATB1 expression in high metastatic potential MHCC-97 H and upregulate the SATB1 expression in low metastatic potential Hep G2 respectively.3.Transwell migration assay and wound-healing assay were used to investigate the metastasis of liver cancer after up-regulation or silencing of the expression of SATB1.Results:1.The result showed that SATB1 expression was significantly higher in hepatocellular carcinoma tissue than in carcinoma-adjacent tissues.2.SATB1 expression was related to tumor size,differentiation degree,hemorrhage and(or)necrosis,metastases or not and the TNM stage of the tumor.3.Both the m RNA and protein expression of SATB1 were higher in MHCC-97 H than Hep G2.4.The migration capability of high metastatic potential MHCC-97 H was weakened after SATB1 silencing,and the migration capability of low metastatic potential Hep G2 was enhanced after SATB1 up-regulating.Conclusion:1.SATB1 expression is positively correlated with the metastasis of liver cancer and that the opposite is also true.2.The metastasis of liver cancer can be regulated by SATB1 expression.3.The detection of SATB1 expression has a reference value for the pathological diagnosis and target therapy of liver cancer. |
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