MicroRNA-141 Regulates The Proliferation,Migration And Oxidative Stress Of HaCaT Cells Via Keap1/Nrf2 Pathway

Background: Diabetic wound healing is a global medical problem,and currently,there is no effective treatment.One of the reasons why diabetes is difficult to heal is the imbalance of oxidative stress.At present,the Keap1/Nrf2 signaling pathway is an important regulatory pathway in regulating oxidative stress.Non-coding RNAs are endogenous single-stranded small RNA molecules that can produce the same biological effects by matching multiple targets.This study focused on exploring whether micro RNA141 could regulate keratinocytes’ oxidative stress process through the Keap1 /Nrf2 signaling pathway in simulated diabetic high oxidative stress state in vitro and further explored its related molecular mechanism providing a new possibility for the treatment of diabetic wounds.The level of oxidative stress and promote the repair of diabetic wounds.Methods: We selected human immortals’ keratinocytes as the research object and simulated the high oxidative stress state of diabetic wounds in vitro by hydrogen peroxide and high glucose medium.RT-PCR was used to detect the changes in mi RNA expression levels under oxidative stress.The activity and proliferation of keratinocytes under oxidative stress were detected by EDU and CCK-8 assay.A scratch test saw the migration of keratinocytes under oxidative stress.DCFH-DA probe,SOD(superoxide dismutase),MDA(malondialdehyde),and GSH-Px(glutathione peroxidase)were used to detect the regulation and protective effect of mi RNA on the level of reactive oxygen species in keratinocytes under oxidative stress.WB and RT-PCR detected the m RNA and protein expression levels of Keap1,Nrf2,and HO-1 after mi RNA transfection.Results: according to the result of the experiment we know,in the condition of oxidative stress,cutin cell proliferation,migration ability significantly lower formation,oxidative stress levels increased significantly,while in transfection mi R-141-3p,inhibits the expression of Keap1,thereby enhancing the expression level of Nrf2 and downstream Ho-1,and promoted the cutin cell migration and proliferation.Conclusions : Micro RNA-141 can regulate the level of oxidative stress in keratinocytes through the Keap1 / Nrf2 signaling pathway,thus increasing the possibility of beneficial to diabetic wound healing and providing a new direction and idea for the treatment of diabetic wound healing.