| Objective: To observe the analgesic effect of Bo NT/A subcutaneous injection of hindpaw and intra-articular injection on sodium monoiodoacetate(MIA)-induced osteoarthritis rats and the effect of P2X4 receptor(P2X4R)expression in the spinal cord.Methods: The model of osteoarthritis was established by injecting 4mg/50 u L monoiodoacetate(MIA)into the ankle cavity of rats.After modeling,20 ul normal saline or5 U /20 u L Bo NT/A was injected into the subcutaneous or articular cavity of the hindlimb ipsilateral to affected side.The pain behavioral tests were performed before and after MIA injection as well as after Bo NT/A injection.The expression level of P2X4 R in the spinal cord was detected by western blot at each time point.Results:Compared with the rats in the sham group,the MIA-induced osteoarthritis rats showed significant decreases in the pain behavioral tests(P < 0.01),the expression of P2X4 R in the spinal cord was significantly upregulated.Compared with the hindpaw skin injection of normal saline group,the subcutaneous administration of Bo NT/A rats showed definite improvement in the pain behavioral tests(P < 0.05).Western blotting results indicated that the subcutaneous of hindpaw administration of Bo NT/A inhibited the expression of P2X4 R in the spinal cord.Compared with the intra-articular injection of normal saline group,the intra-articular injection of Bo NT/A rats showed significant improvement in the pain behavioral tests(P<0.05),and P2X4 R protein expression level in the spinal cord of the rats was significantly decreased(P<0.05).Conclusion: Subcutaneous and intra-articular injection of Bo NT/A could reduce the expression of P3X4 R in the spinal cord and alleviate the pain of osteoarthritis rats. |
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