To Explore The Feasibility Of Repairing The Wound By Human Amniotic Membrane Combined With Umbilical Cord Mesenchymal Stem Cells

BackgroundAt present,the main treatment for patients with large area skin defects and chronic wounds is still autologous skin graft.However,we still should pay more attention to the relatively long treatment time window,the shortage of donor site,the high cost of treatment and the quality of life after operation.We know that the change of tissue engineering skin technology has always been the development direction of wound repair.Therefore,it is necessary to find a kind of wound covering with cell activity,which can repair the wound faster and better,and reach the physiological state of normal skin.ObjectiveThe purpose of this study is to construct a tissue-engineered skin with cellular activity by amniotic membrane combined with human umbilical cord mesenchymal stem cells,and to provide a new idea for clinical wound repair patients.MethodsHuMSCs were isolated from Wharton glue of human umbilical cord,amplified and subcultured to the third generation,and then inoculated in vitro to the surface of fresh chorionic amniotic membrane matrix.HuMSCs group,amniotic membrane group and amniotic membrane combined with HuMSCs group were cultured in specific medium respectively.The cell activity was identified on the 3rd and 14 th day of culture,and the histological condition of each group at different time periods was observed under microscope.The concentration of growth factor in the supernatant was detected by enzyme-linked immunosorbent assay(ELISA),and histological verification was made.ResultsAfter 14 days of culture,HuMSCs inoculated on amniotic membrane could maintain cell activity and secrete more growth factors than HuMSCs group and amniotic membrane group.HE staining showed that there were a large number of HuMSCs in the amniotic membrane matrix layer in the amniotic membrane combined with HuMSCs group.There are a lot of collagen and hyaluronic acid.ConclusionsAmniotic membrane can be used as a good culture medium for HuMSCs in vitro and increase the expression levels of growth factors in HuMSCs.Amniotic membrane combined with HuMSCs is expected to provide a new idea for clinical wound repair.