Analysis Of The Clinical Value For Anti-Ro52 Antibody And Anti-Ro60 Antibody In Connective Tissue Disease

Objective:To investigate the expression of anti-Ro52 antibody and anti-Ro60 antibody,the clinical and other serological characteristics in patients with different connective tissue disease(CTD),especially their clinical significance in CTD-interstitial lung disease(ILD),in order to clarify the diagnostic and prognostic value of anti-Ro52 antibody in CTD.Methods:A retrospective analysis was performed from October 2019 to January 2021,785 cases were collected from the Department of Immunology and Rheumatology,the Second Hospital of Hebei Medical University.These patients were diagnosed with CTD including primary Sjogren’s syndrome(p SS),polymyositis(PM)/dermatomyositis(DM),rheumatoid arthritis(RA),systemic lupus erythematosus(SLE),and systemic sclerosis(SSc).They were divided into different groups according to the expression of anti-Ro52antibody and anti-Ro60 antibody.The general data,clinical symptoms and laboratory tests were collected and analyzed.The risk factors of CTD combined with ILD were discussed.Results:1.According to the negative or positive results of anti-Ro52 antibody and anti-ro60 antibody,CTD patients were divided into four groups,including Ro52~+Ro60~-group(group A),Ro52~-Ro60~+group(group B),Ro52~+Ro60~+group(Group C)and Ro52~-Ro60~-group(Group D).(1)There were statistically significant difference in age and gender among different groups(P<0.05);(2)As for clinical symptoms,there were statistically significant differences in the probability of joint swelling and pain,myalgia/myasthenia,dry mouth/dry eyes and chest tightness/shortness of breath among different groups(P<0.05);(3)Inflammatory indicators:C-reactive protein(CRP),erythrocyte sedimentation rate(ESR)and serum ferritin(SF)were statistically significant(P<0.05);(4)The result of cytokines:the levels of IL-2,IL-4,IL-5,IL-6,IL-10,IL-17,IL-12p70,IFN–αand IFN–γwere statistically significant(P<0.05),while the expression of IL-1β,IL-8 and TNF-αwere not statistically significant(P>0.05);(4)Immunological indexes:there were significant differences in the levels of immunoglobulin(Ig)G,Ig M and complement C3,C4(P<0.05),while the statistical difference of Ig A was no significance(P>0.05).The differences of antinuclear antibody(ANA),anti-SSB antibody,anti-CCP antibody and rheumatoid factor(RF)were statistically significant(P<0.05).2.The CTD patients were divided into two groups,including CTD combined with ILD(CTD-ILD)group and CTD without ILD group.Logistic stepwise regression analysis was done,we found that the probability of ILD in male increased by 56.7%when compared with female.The risk of ILD increased by 3.8%for every additional year of age;According to the analysis of antibody expression,it was found that the probability of ILD in Ro52~-Ro60~+patients reduced by 60.7%,the probability of ILD in Ro52~+Ro60~+patients decreased by 70.2%,and the probability of ILD in Ro52~-Ro60~-patients reduced by 65.4%when compared with Ro52~+Ro60~-patients.Conclusions:1.Anti-Ro52 antibodies can be found in a variety of autoimmune diseases and are closely related to interstitial lung diseases.CTD with anti-Ro52 antibody positive should be highly vigilant for the occurrence of ILD.2.These factors,including male,advanced age and anti-Ro52 antibody positive,are independent risk factors of ILD in CTD patients.3.The double positive of anti-Ro52 antibody and anti-Ro60 antibody is more common in SS,and the synergistic analysis of anti-Ro52 antibody and different antibodies is more instructive for clinical diseases.