Preliminary Study Of Permeable Nanoparticles For The Relief Of Diabetic Cataract

Diabetic cataract(DC)is a common diabetic ocular complication,which develops rapidly,and the crystalline lens may be turbid in a short time,leading to blindness that has a tremendous influence on the drowning individuals’ life and health.At present,the main methods of cataract treatment are surgery and drug intervention,but surgical treatment is prone to serious postoperative complications,including choroidal detachment;pupillary block;persistent corneal edema;retinal detachment and so on.Traditional drug treatment is administered by injection or eye drops.The injection of drugs causes great discomfort and pain leading to poor patient compliance.The current eye drops have problems such as low antioxidant properties,which severely limit the effective use of drugs.In response to the above problems,this article designed a kind of antioxidant and anti-glycated nanoparticle as eye drops with corneal permeability for non-invasive and painless relief of diabetic cataract in rats.The specific research contents are as follows:(1)Preparation and characterization of permeable nanoparticles DTPNCs.The surface of Pt nanoclusters(Pt NCs)can be coated with TAT-conjugated dextran amphiphilic polymer(DTAT)by electrostatic adsorption under the action of ultrasound to form DTPNCs with corneal permeability.The particle size of DTPNCs was about160 nm and the effective zeta potential was positive.And DTPNCs exhibited excellent stability without significant differences of the diameter and zeta potential after being kept at 4 ℃ for over 30 days.Due to the nano-enzyme-like activity of Pt NCs,DTPNCs possessed antioxidant properties.The combination of amphiphilic polymer DTAT and dispersed in 0.1% Pluronic F127(0.1% F127)made DTPNCs own excellent corneal permeability.(2)Antioxidant capacity and the evaluation of antiglycation activity of permeable nanoparticles DTPNCs in vitro.The results indicated that permeable nanoparticles DTPNCs exhibited better security to HLE-B3 and played an antioxidant protective role in the process of lens epithelial cell oxidative damage,and could inhibit reducing sugar-induced α-crystallin glycation and cross-linking.(3)Distribution in the eyes and safety of permeable nanoparticle DTPNCs as eye drops.The results displayed that DTPNCs by topical administration of eye drops had the ability of corneal permeation and further reaching the aqueous humor and the lens,and then protected the lens from oxidative damage and glycation.And permeable nanoparticles DTPNCs displayed good biocompatibility and low toxicity in vivo,which could be used in subsequent treatment experiments of diabetic rats.(4)Permeable nanoparticles DTPNCs as eye drops for relief of diabetic cataract in rats.We successfully established streptozotocin(STZ)-induced diabetic rat models by IV injection.The results showed that after administration of high concentrations of DTPNCs eye drops,the stage of the cataract was obviously delayed.Further biochemical analysis of the lens showed that there was significant decrease of the concentration of Malondialdehyde(MDA)and carbonyl products,and remarkable increase of glutathione(GSH).And the content of pentosidine(one of the AGEs)declined.In summary,antioxidant and anti-glycated nanoparticle DTPNCs with corneal permeability were carried out by topical administration of eye drops for non-invasive and painless relief of diabetic cataract in rats,which create innovative applications of Pt NCs in the field of ophthalmology,providing an idea for treatment of other eye diseases.