Study On The Effect Of Caloric Restriction On Ferroptosis In The Kidney Of Aged Mice

Objective:Observe the effect of caloric restriction on ferroptosis in the kidney tissue of aged mice,and explore the possible mechanism.Methods:Sixteen healthy 12-month-old male C57BL/6 mice were selected and randomly divided into two groups(n=8): the elderly normal diet group(A+AL)and the elderly calorie restriction group(A+CR);healthy 3-month-old mice were selected Male C57BL/6 mice were taken as(n=8): young free diet group(Y+AL).ATfer calorie restriction in the calorie restriction group,Three groups were given automatic biochemical analyzer to detect blood creatinine and urea nitrogen levels;iron ion content and glutathione peroxidase(GSH-PX)activity in mouse kidney tissue were measured by colorimetric method;measured by TBA Kidney tissue MDA content;microplate method to detect kidney tissue glutathione(GSH)content;Western blot method to detect transferrin(Tf),transferrin receptor(Tf R),and glutathione in kidney tissue Peroxidase 4(GPX4)protein expression level.Results:Compared with Y+AL,A+AL serum creatinine and urea nitrogen content did not change significantly(P>0.05);iron ion level was significantly increased(P<0.05);GSH and GSH-PX content was significantly reduced(P<0.05);MDA content increased significantly(P>0.05);Tf and Tf R protein expression increased significantly(P<0.05);GPX4 protein expression decreased significantly(P<0.05).Compared with Y+AL,serum creatinine and urea nitrogen levels of A+CR were significantly reduced(P<0.05);iron ion levels were significantly increased(P<0.05);GSH and GSH-PX levels were significantly reduced(P<0.05);MDA content increased significantly(P<0.05);Tf and Tf R protein expression increased significantly(P<0.05);GPX4 protein expression decreased significantly(P<0.05).Compared with A+AL,serum creatinine and urea nitrogen content of A+CR were significantly reduced(P<0.05);iron ion levels were significantly reduced(P<0.05);GSH and GSH-PX levels were significantly increased(P<0.05);MDA content was significantly reduced(P<0.05);Tf and Tf R protein expression were significantly reduced(P<0.05);GPX4 protein expression was significantly increased(P<0.05).Conclusion:Calorie restriction can improve kidney ferroptosis in aging mice,and its mechanism may be through regulation of iron imbalance and oxidative damage.