S1P Phosphatase 2 Regulates The Key Genes Of Pancreatic β-cell Proliferation And Endoplasmic Reticulum Stress Screening And Bibliometric Analysis

Objective:Explore the key genes of S1P phosphatase 2(S1P phosphatase 2,SPPase2)that regulate pancreatic β-cell growth and endoplasmic reticulum stress.And use bibliometrics methods to explore the development trends and research frontiers in key gene research fields.Methods:Obtain the GSE73131 gene chip data set from the GEO database,screen the differentially expressed genes between SPPase2 knockout mice and wild-type mice,construct a Pro-tein-Protein Interaction(PPI),and use STRING and Cytoscape software for modularization analysis and lock key genes.By analyzing relevant documents retrieved from the Web of Science scientific expansion database from 1986 to 2020,using CiteSpace and VOSviewer software to conduct bibliometrics co-occurrence analysis of key gene-related documents on countries,institutions,authors,keywords,etc.Results:Compared with wild-type mice,a total of 150 up-regulated genes and 30 down-regulated genes were screened in the SPPase2 knockout group.The functions involved are mainly in type 1 diabetes,chemokine signaling pathways,cytokines and cells,interaction of factor receptors,cell adhesion molecules,intestinal immune network that produces IgA.After modular analysis,a pivotal gene was finally determined,namely CD48 gene.Bibliometric analysis of the CD48 gene found that the number of publications in the past ten years has basically maintained an average annual increase of 20-30.The United States(n=115,43.7%),China(n=32,12.2%),and Germany(n=29,11.0%)are the three countries with the largest global contributions to the field of CD48 gene research.In addition,Harvard University(n=15,the United States),the Hebrew University of Jerusalem(n=14,Israel),and the U.S.Food and Drug Administration(n=9,the United States)are the top three institutions in terms of publication volume.CD48-related research cell types over time are natural killer cells,basophils,and phagocytes around 2012,red blood cells,platelets,and stromal cells around 2014,and hematopoietic stem cells around 2016,As well as blood white blood cells around 2018 and recent autophagy cells in 2020.Conclusion:For diabetes caused by abnormal sphingolipid metabolism,CD48 gene may be a potential research and therapeutic target gene.At present,CD48 research is more adequate in blood system diseases than other systems.Research on other endocrine systems such as diabetes is rarely carried out,so the research potential is great.