The Effects Of FGBβ-15-42 Peptide On Mitochondrial Autophagy-related Proteins In Ischemia-reperfusion AKI Rats

Objective:To investigate the effect of FgBβ15-42 peptide on mitochondrial autophagy-related proteins in ischemia-reperfusion AKI rats.Methods:(1)Experimental grouping: SD rats(male)were randomly divided into sham operation group,model group,FgBβ15-42 peptide treatment group,and FgBβ15-42 peptide+chloroquine group(n=8).Ischemia reperfusion model modeling method:noninvasive artery clamp closed bilateral renal arteries,blood and renal tissue samples were collected for 24 hours after reperfusion.(2)Serum creatinine(Scr)and urea nitrogen(BUN)levels were detected;The pathological changes of renal tubular were observed after HE staining,and the pathological injury of renal tubular in each group was analyzed.(3)The expressions of apoptosis-related protein Bcl-2,autophagy related protein LC3 and P62 in renal tissue were detected.Results:(1)Compared with sham operation group,SCR and BUN levels in model group,FgBβ15-42 peptide treatment group and FgBβ15-42 peptide + chloroquine group were significantly increased(P < 0.01),especially in model group.Compared with model group,SCR and BUN levels in FgBβ15-42 peptide treatment group were significantly decreased(P < 0.01),and the difference was statistically significant.(2)Compared with control group,model group,FgBβ15-42 peptide treatment group,FgBβ15-42 peptide + chloroquine group rat renal tubular epithelial cells are damaged,different level model group is the most significant.The degree of renal tubular injury in FgBβ15-42 peptide treatment group was significantly less than that in model group.Compared with sham operation group,pathological injury scores in each group were significantly increased(P < 0.01);Compared with the model group,the pathological injury fraction in FgBβ15-42 peptide treatment group was significantly decreased(P <0.05).(3)Compared with the sham operation group,the expression level of Bcl-2 protein in the model group was significantly decreased(P < 0.01),showing a statistically significant difference;At the same time,compared with the model group,the protein expression level of Bcl-2 in FgBβ15-42 peptide treatment group was significantly increased(P < 0.05),with statistical difference.(4)After renal injury caused by renal ischemia reperfusion in rats,autophagy-related protein LC-3 II/I in renal tissue of rats in model group was significantly decreased(P <0.05),with statistical difference,while P62 was significantly increased(P <0.05).Compared with model group,autophagy-related protein LC-3 II/I was significantly increased(P < 0.01)and P62 was significantly decreased(P < 0.01)in FgBβ15-42 peptide treatment group,with statistical differences.Conclusion:(1)24 hours after renal IRI injury,autophagy showed a trend of weakening,and FgBβ15-42 peptide had a good protective effect on renal IRI.(2)The protective effect of FgBβ15-42 peptide on renal IRI may be realized by enhancing mitochondrial autophagy.(3)Further studies are needed to determine whether the protective effect of FgBβ15-42 peptide on renal IRI is realized by reducing cell apoptosis.