SIRT1 Participate In The Protective Effects Of Sevoflurane Postconditioning On Learning And Memory Impairment In Hemorrhagic Shock And Resuscitation Mice

Objective To explore the role of the silent information regulator(silent information regulation 1,SIRT1)-mediated apoptotic pathway in the protection of sevoflurane postconditioning in hippocampal neuronal injury of mice induced by hemorrhagic shock resuscitation(HSR).Methods A mouse model of HSR was established.The male C57BL/6J mice were randomly divided into: sham operation group(Sham group),HSR group(Shock group),sevoflurane treatment group(Sevo group),sevoflurane combined with SIRT1 specific inhibitor treatment group(EX527+Sevo group)and EX527 treatment group(EX527group).The volume of cerebral infarction was detected by TTC staining method.TUNEL staining method detects the changes of hippocampal nerve cells in each group of mice,The learning and memory abilities were detected by Morris water maze test.The expression of SIRT1 and apoptosis-related protein levels were detected by Western blot analysis.Results The latency of the model mice reaching the platform in the Morris water maze test was prolonged,while the movement distance in the target quadrant was similarly reduced.Besides,the cerebral infarction volume was remarkably increased,The number of TUNEL staining positive cells increased,and the expression of SIRT1 and Bc1-2 was increased while the pro-apoptosis protein Bax,and cleaved-Caspase 3 expression level was increased;sevoflurane treatment improved nerve injury in HSR.After combined treatment with sevoflurane and SIRT1 inhibitor EX527,the protective effect of sevoflurane was attenuated on nerve injury in HSR.Conclusion Sevoflurane postconditioning may play a protective role against hippocampal neuronal injury caused by HSR mediated by SIRT1 apoptosis-related pathway.