Bioinformatics Analysis And Identification Of Dysregulated Genes Associated With Lupus Nephritis In Renal Tissue

Objective:Lupus nephritis(LN)is a complex organ inflammation mediated by immunity,and its pathogenesis remains unclear.In this paper,we use GEO(Gene Expression Omnibus)database to conduct bioinformatics analysis on the whole gene transcription data of kidney tissues of patients with LN,and mine the genes,signal pathways and transcription factors related to LN,which will lay a molecular foundation for the future research on LN.Methods:1.Combine the GSE99339 and GSE32591 data sets in the GEO database to perform LN-related differential gene expression analysis and intersection analysis.2.Use Metascape tool to perform GO annotation and KEGG enrichment analysis on LN-specific expression genes.3.Use the STRING database to identify protein interactions of specific genes and use the Cytoscape MCODE plug-in to screen key gene modules.4.Through the KEGG,Reactome,HALLMARK signal pathway database,the LN-specific genes were enriched and analyzed,and the key signal pathways were screened out.5.Use the TRUUST database to find transcription factors that regulate key signal pathways.Results:1.Compared with the healthy control group and other kidney disease control groups,the LN group has a different number of differentially expressed genes.The intersection analysis yielded 32 differentially expressed genes.These 32 genes were significantly up-regulated in the LN group and down-regulated in the control group,so they are LN-specifically expressed genes.2.LN-specifically expressed genes are mainly enriched in interferon-related pathways,hepatitis C,influenza A,measles,Epstein-Barr virus infection and other viral infection processes.3.The Cytoscape MCODE screens out a key gene module in the protein interaction network of LN-specifically expressed genes.4.The genes of the key gene modules are significantly enriched in interferon α,β and γ pathways.5.The TRUUST database shows that STAT1 and IRF1 are the key transcription factors of the three signal pathways.Conclusion:1.Compared with the control group,there were a total of 32 significantly up-regulated differentially expressed genes in renal tissue of patients in the LN group.2.LN-specific genes are mainly enriched in the process of multiple virus infections,and there may be a certain connection between virus infection and LN.3.The key signal pathways enriched by LN-specific expression genes are interferon α,β and γ pathways.4.STAT1 and IRF1 are key transcription factors in the interferon signaling pathway.The regulation of STAT1 and IRF1 may become a new target for the treatment of LN.