| Objective: Emergency coronary bypass grafting is an effective method to treat acute myocardial infarction.It is mainly divided into two ways: non cardiopulmonary bypass and cardiopulmonary bypass.Ischemia-reperfusion injury is one of the important factors that affect the prognosis of acute myocardial infarction.In this study,cardiac arrest and non-cardiac arrest were used to study the myocardial ischemia-reperfusion injury after acute myocardial infarction in rats.Using metabonomic research platform to explore the metabolites and metabolic pathways of different ways of ischemiareperfusion injury.Methods: 40 male S-D rats weighing from 250 g to 400 g were randomly assigned to Sham group,AMI group,NCA group and CA group with 10 rats in each group.After anesthesia,tracheal intubation was inserted from the incision of trachea.Mechanical ventilation was performed rapidly after median chest incision.In Sham group,the anterior descending branch was not ligated by suture needle.In the other three groups,the anterior descending branch was ligated for 45 minutes to establish acute myocardial infarction model.The perfusion needles were inserted into the right carotid artery in all groups.In the CA group,4 ℃ HTK solution was injected through the right carotid artery,resulting in cardiac arrest,and the rat heart was harvested and placed in 4 ℃ HTK for 30 minutes.Sham group,AMI group and NCA group were perfused with KH solution through carotid artery exchange perfusion technology to Langendorff perfusion system for 30 minutes.Then,in AMI group,the anterior descending branch was ligated and the KH solution was infused continuously for 15 minutes.The other three groups were perfused with KH solution for 15 minutes under the condition of reopening the anterior descending branch.ECG,blood pressure and coronary flow were recorded during reperfusion.After 15 minutes of reperfusion,the heart was examined by pathological section,myocardial water content and metabonomics.Result: Reperfusion arrhythmias were found in NCA group and Cr group,but not in Sham group and AMI group.NCA group was better than AMI and CA group in the left ventricular systolic pressure,left ventricular development pressure and the maximum rate of rise of left ventricular development pressure.The coronary flow of NCA group was higher than that of CA group.The myocardial water content of Sham group was lower than that of the other three groups.Except Sham group,the other three groups showed dark stained areas under Heidenhain staining.In metabonomic,25 metabolites were screened out,including 21 metabolites,which including three types of lysophosphatidylcholine,seven types of lysophosphatidylethanolamine,two types of lysophosphatidylcholine,glycerophosphatidylcholine,oleamide,octadecylamine,1,2,4-Nonadecanetriol,2-Methylene-4-oxopentanedioic acid,Geranylcitronellol,1-Pentanesulfenothioic acid,Pristanic acid,(1x,2x)-Guaiacylglycerol 3-glucoside.The m/z of four unidentified substances was 313.273,119.085,266.284,199.991,respectively.Three Lysophosphatidylcholines,two lysophosphatidic acids,Octadecylamine and Glycerophosphocholine were up-regulated in AMI group and NCA group,and down regulated in CA group;seven kinds of lysophosphatidylethanolamine,oleamide,1,2,4-Nonadecanetriol,Geranylcitronellol and Pristanic acid were up-regulated in each group,2-Methylene-4-oxopentanedioic acid,1-Pentanesulfenothioic acid and 1x,2x)-Guaiacylglycerol 3-glucoside were down regulated in each group.The m/z ratio substances was 313.273,119.085 and 266.284,which was up-regulated in each group,while the m/z ratio was 199.991,which was down regulated.The m / z of 119.085 was down-regulated in NCA group,up-regulated in AMI and CA group.The m/z was 313.273,266.284,which were up-regulated in each group,while the m/z was 199.991,which was down regulated.Four metabolic pathways were screened: glycerophospholipid metabolism glycerolipid metabolism,phosphatidylinositol signaling system,ether lipid metabolism.Conclusion: In this study,a rat model of AMI was successfully established.The models of myocardial ischemia-reperfusion injury under the condition of cardiac arrest and non cardiac arrest were simulated.25 Characteristic Metabolites and 4 metabolic pathways were screened by metabonomics.It may provide a new therapeutic target and research direction for the following research. |
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