| Objective:The study established a rat model of osteoporosis,applied adipose-derived mesenchymal stem cells locally to the vertebral body,and observed the repairing ability of adipose-derived mesenchymal stem cells on osteoporotic vertebral bodies through imaging analysis and pathological observation.Provide an animal experimental basis for the local application of adipose-derived mesenchymal stem cells to treat osteoporotic vertebral bodies.Method:Twenty 8-week-old female SD rats were selected,and the osteoporosis model was constructed by ovariectomy and steroid administration.They were randomly divided into 2 groups.Adipose-derived mesenchymal stem cells or PBS were injected into the lumbar 5 of rats in the experimental group and control group via abdominal approach.Two and four weeks after the operation,the experimental animals were sacrificed.The surgical segments were completely removed.Analysis of lumbar spine defect healing was performed by radiographic and histological observation.Results:Osteoporosis rats were successfully constructed.According to the plan,10 rats in the experimental group and the control group were obtained.Micro-CT imaging evaluation: the trabecular thickness and degree of anisotropy in the experimental group at 2 weeks,and the trabecular thickness,trabecular separation and Bone Volume/Total Volume in the experimental group at 4 weeks were different from those in the control group(P<0.05).The trabecular number and degree of anisotropy in the experimental group at 2 weeks and 4 weeks showed no statistical significance compared with the control group(P>0.05).Histological observation: He staining in both the experimental group and the control group showed destruction of bone trabecular structure,reduction of bone marrow cells,and visible fibrous connective tissue and osteoblasts.Conclusions:In animal experiments in rats,the improvement of vertebral osteoporosis can be observed after local injection of adipose derived mesenchymal stem cells. |
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