Correlation Analysis Between Peripheral Blood T Cell Subsets And Disease Activity Of Systemic Lupus Erythematosus And Lupus Nephritis

Objectives:This study was performed to analyze the correlation and to explore the relationship between the Number of Peripheral Blood CD4+/CD8+T Cells and different disease activity in patients with systemic lupus erythematosus(SLE).Methods:In this retrospective observational study,folders of 98 patents with SLE seen in the Zhujiang Hospital affiliated to Southern Medical University between January 2018 and December 2020 were retrospectively analyzed,including clinical features and laboratory test results.SPSS 24.0 statistical software was used to analyze and process the data.Results:(1)ninety-eight patients with SLE had been evaluated,among which were 9 males and 89 females.The mean age was 38.02±13.63 years.The mean course of disease was 24(3,96)months.(2)The CD4+T cell count and CD8+T cell count in the high-activity group and the low-activity group were significantly lower than the stable group,with statistically significant differences(P<0.01).CD4+T/CD8+T in the high-activity group and the low-activity group was lower than the stable group.There was no statistically significant difference between groups(P>0.05).The levels of hemoglobin,lymphocyte count,platelet,albumin,complement C3,and complement C4 in the high-activity group and the low-activity group were significantly lower than the stable group,and the SLEDAI score and the positive rate of anti-dsDNA antibody in the high-activity group and the low-activity group were higher.We found statistically significant differences between groups(P<0.01).(3)The CD4+T cell count and CD8+T cell count in the lupus nephritis(LN)group were higher than the non-LN group,with statistically significant differences(P<0.05).CD4+T/CD8+T in the LN group was lower than the non-LN group,with statistically significant difference(P<0.05).The levels of albumin and complement C3 in the LN group were lower than the non-LN group,and creatinine and 24 hours urinary protein excretion in the LN group were higher.We found statistically significant differences between groups(P<0.05).(4)The CD4+T cell count and CD8+T cell count in the active LN group were lower than the inactive LN group,with statistically significant differences(P<0.05).CD4+T/CD8+T in the active LN group was lower than the inactive LN group.There was no statistically significant difference between groups(P>0.05).The levels of hemoglobin,lymphocyte count,albumin,complement C3,and complement C4 in the active LN group were lower than the inactive LN group,and the SLEDAI score,creatinine,24 hours urinary protein excretion and the positive rate of anti-dsDNA antibody in the active LN group were higher.We found statistically significant differences between groups(P<0.05).(5)The CD4+ T cell count was negatively correlated with SLEDAI score(r=-0.597,P<0.01),24 hours urinary protein excretion(r=-0.318,P<0.01),but was positively correlated with complement C3 levels(r=0.311,P<0.01).The CD8+T cell count was negatively correlated with SLEDAI score(r=-0.467,P<0.01),but was positively correlated with complement C3 levels(r=0.235,P=0.020),complement C4 levels(r=0.220,P=0.029).CD4+T/CD8+T was negatively correlated with 24 hours urinary protein excretion(r=-0.331,P<0.01).Conclusions:(1)The CD4+T cell count is negatively correlated with SLE disease activity.(2)Decreased CD4+T cell count in active LN is correlated with 24 hours urinary protein excretion.(3)SLE patients have T cell subsets abnormalities.CD4+T/CD8+T is correlated to 24 hours urinary protein excretion.