MiR-140-3p Contributes To Osteoporosis By Targeting Pten And Activating The PTEN/PI3K/Akt Signaling Pathway

Objective:By studying that targeting mi R-140-3p affects the expression of signaling pathways,it provides a new basis for the treatment of osteoporosis.Materials and methods:Osteoporosis is a disorder of bone metabolism,a systemic disease that severely affects the quality of life of the elderly and postmenopausal women.Healthy volunteers and postmenopausal patients with OP were included in the study.Bone mineral density(BMD)was detected by dual energy X-ray absorptiometry(DXA).Osteoclast differentiation and osteoblast differentiation were induced by culturing CD14 PBMCs and C2C12 cells,respectively.The interaction between mi R-140-3p and PTEN was predicted and validated by targetscan7.2 and dual luciferase reporter assay,respectively.mi RNA /RNA levels and protein levels were determined by quantitative real-time polymerase chain reaction(QRT-PCR)and Western blot,respectively.Cell proliferation and apoptosis were detected by 5-ethynyl-2 ’-deoxyuridine(EDU)staining and flow cytometry,respectively.Cellular differentiation of CD14 + PBMC and C2C12 cells was examined by tartrate resistant acid phosphatase staining and alizarin red staining,respectively.Alkaline phosphatase activity was measured by the alkaline phosphatase method.Results:Differences in age,body mass index(BMI)and bone mineral density(BMD)were observed between the OP and control groups.Compared with the control group,the levels of mir-140-3p in PBMCs were increased and the levels of PTEN were decreased in the OP group.There was a negative correlation between the levels of mir-140-3p and PTEN in the serum of OP group.Mir-140-3p down regulated the expression of PTEN by targeting.Mir-140-3p inhibitors inhibited cell proliferation,differentiation,and promoted apoptosis in CD14 + PBMCs by targeting PTEN and inactivating the PTEN / PI3 K / Akt signaling pathway,while promoting proliferation,differentiation,and inhibiting apoptosis in C2C12 cells.These findings suggest a potential therapeutic role of mir-140-3p in the treatment of patients with OP.Conclusion:1.The pathophysiological mechanism leading to osteoporosis is very complex.At present,one or more of the molecular pathways leading to osteoporosis can be selected for combined drug treatment in clinic,so as to artificially regulate the level of BMD in the body and provide a new way for the prevention of osteoporosis.2.miR-140-3p has broad prospects in the treatment of OP patients...