| Objective:We tested the contents of hypoxia inducible factor-1α,transforming growth factor-β1and α-smooth muscle actin in serum of patients with chronic kidney disease,aiming to investigate the effect of hypoxia inducible factor-1α on renal fibrosis.Method:Selected from August 2020 to February 2021 in the Department of Nephrology,First Hospital of Jilin University,120 patients were diagnosed with CKD.According to the MDRD formula,the estimate glomerular filtration rate(e GFR)was calculated and these patients were divided into CKD1-5 stages,there are 30 cases in CKD1-2 stages and 30 cases in other stages.Meanwhile,the structure of the normal control group is 30 healthy people.Collecting the age,sex,weight,past medical history,serum creatinine(Scr),urea nitrogen(BUN),serum albumin(ALB),peripheral red blood cell count(RBC),hemoglobin(HB)and other clinical indicators.The enzyme-linked immunosorbent assay method was used to detect the levels of HIF-1α,TGF-β1 and α-SMA in serum.Analyze the connection between HIF-1α in serum of CKD patients of different stages and various related factors by statistical software,so as to explore the effect of HIF-1α on renal fibrosis.Results:(1)Compared with the healthy control group,there were significant differences in the serum of Scr,BUN,e GFR,HB and RBC in CKD patients of 3-5 stages(P<0.05).And with the increase of CKD staging,Scr and BUN gradually increased,and e GFR,HB and RBC gradually decreased,the difference was statistically significant(P<0.05).The level of ALB in serum of CKD1-5 patients was statistically significant compared with healthy controls(P<0.05).(2)The levels of TGF-β1 in CKD1-2,CKD3,CKD4,and CKD5 stages were 9.78(9.14,10.14)μg/L,10.03(9.25,11.26)μg/L,11.08(10.21,12.93)μg/L and 15.52(12.82,17.77)μg/L respectively,the content of TGF-β1 in the serum of the healthy control group was 7.44(6.36,8.59)μg/L.Compared with the healthy control group,the TGF-β1content in peripheral blood of CKD patients of different stages increased significantly,and the difference was statistically significant(P<0.05),the level of TGF-β1 in CKD4 stage was significantly higher than that of CKD1-2,and the level of TGF-β1 in CKD5 stage was significantly higher than that of CKD in all stages,the difference was statistically significant(P<0.05),And the level of TGF-β1 showed a gradually increasing trend among CKD groups.(3)The levels of α-SMA in CKD1-2,CKD3,CKD4 and CKD5 stages were 97.86(75.58,119.18)ng/L,110.46(81.15,129.84)ng/L,122.57(102.71,170.29)ng/L and 222.37(171.26,269.36)ng/L,the content of α-SMA in the serum of the healthy control group was65.89(47.96,76.30)ng/L.Compared with the healthy control group,the serum of α-SMA content in CKD patients of 1-3 stages increased significantly,and the difference was statistically significant(P<0.05).Among them,the level of α-SMA in CKD5 stage was significantly higher than that of CKD in all stages,the difference was statistically significant(P<0.05),And the level of α-SMA showed a gradually increasing trend among CKD groups.(4)The levels of HIF-1α in the serum of CKD1-2,CKD3,CKD4,and CKD5 stages were respectively 47.17(40.13,62.16)ng/L,51.09(39.72,68.98)ng/L,71.98(41.79,83.77)ng/L and 103.62(79.32,115.00)ng/L;the content of HIF-1α in the serum of the healthy control group was 36.20(18.63,46.13)ng/L.Compared with the healthy control group,HIF-1α levels in CKD patients of 1-3 stages were significantly increased,and the difference was statistically significant(P<0.05).Among them,the level of HIF-1α in CKD5 stage was significantly higher than that in CKD1-2 and CKD3 stages,the difference was statistically significant(P<0.05),And the level of HIF-1α showed a gradually increasing trend among CKD groups.(5)Correlation analysis between serum HIF-1α levels and clinical indicators in CKD patients of different stages: HIF-1α levels are positively correlated with BUN and Scr levels(r=0.433,0.478,P<0.01),and negatively correlated with e GFR levels(r=-0.476,P<0.01);negatively correlated with HB and RBC levels(r=-0.215,-0.238,P<0.01).(6)Correlation analysis of serum HIF-1α levels and fibrosis indicators in CKD patients of different stages: the levels of HIF-1α are significantly positive correlated with TGF-β1(r=0.575,P<0.01);they have a low degree of positive with α-SMA Correlation(r=0.459,P<0.01).Conclusions:(1)The level of HIF-1α in the peripheral blood of CKD patients is significantly higher than that of normal people,and with the continuous progress of CKD,the level of HIF-1αgradually increases,it is suggested that HIF-1α can be used as one of the indicators to evaluate CKD.(2)The contents of TGF-β1 and α-SMA in peripheral blood of patients with different stages of CKD gradually increase with the increase of CKD stages,indicating that as CKD progresses,the degree of renal fibrosis gradually increases.(3)The levels of HIF-1α in peripheral blood of CKD patients of different stages are positively correlated with TGF-β1 and α-SMA,suggesting that HIF-1α may up-regulate the expression of TGF-β1 and α-SMA proteins,thereby participating in renal fibrosis in CKD patients The process of occurrence and development. |
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