Effect Of Whole Body Vibration Exercise Time On Insulin Resistance In Skeletal Muscle Of Type 2 Diabetic Mice And Its Mechanism

ObjectiveTo investigate whether whole body vibration exercise can improve the level of autophagic activity of skeletal muscle in Type 2 diabetic mice,thus improve insulin resistance and glucose metabolism of skeletal muscle.And whether the length of the vibration makes a difference.MethodsEight-week-old C57BL/6J mice model 180 type 2 diabetic mice,were randomly divided into a quiet control group(4 w、8w、12w)and vibration motion group(4 w、8w、12w).30 mice per group,Type 2 diabetic control group was given type 2 diabetic diet.A vibration motion group with a frequency of 15 Hz,30分钟utes/times,1 per day,6 days/week,There were 4 weeks,8 weeks and 12weeks respectively.After 48 h of the last exercise,Sugar tolerance test(OGTT),insulin tolerance test(ITT);Blood serum was prepared from mouse eyeball to detect venous blood glucose and plasma insulin;Bilateral gastrocnemius HE staining,Western Blot detection of IRS、p-IRS、Akt、p-Akt、P62、Beclin-1、LC3BⅡ/Ⅰ、Glu T4 protein expression levels,immunofluorescence detection of LC3BⅡ/Ⅰ、Glu T4 protein expression levels.Results1.The area under OGTT curve(AUC),ITT curve(AUC)and HOMA-IR in the vibration exercise group were significantly lower than those in the quiet group,The protein expression levels of p-IRS,p-Akt,Beclin-1,LC3BⅡ/Ⅰ,Glu T4 in skeletal muscle of type 2 diabetes mellitus vibration exercise group were significantly higher than those of type 2 diabetes mellitus quiet group.2.Compared with the 8W and 4W exercise groups,the area under the OGTT curve(AUC),the area under the ITT curve(AUC)and the HOMA-IR in the 12 W vibration exercise group were significantly lower,The protein expression levels of p-IRS,p-Akt,Beclin-1,LC3BⅡ/Ⅰ,Glu T4 in skeletal muscle of type 2 diabetes 12 W vibration exercise group were significantly higher than those of type 2 diabetes 8W and 4W exercise groups.Conclusions1.Whole body vibration exercise can increase the level of autophagy in Type 2 diabetic mice,thus mediating insulin resistance and Promoting Skeletal Muscle Glucose metabolism.2.Within a certain time frame,The longer the whole body vibration exercise time,the higher the level of autophagy in type 2 diabetic mice,so as to better improve insulin resistance and promote skeletal Muscle Glucose metabolism.