Effects Of Dietary Addition Of Porphyra,Methionine And Cysteamine On Yolk Taurine Content,Egg Quality And Egg Production In Gallus Domestiaus

This test first established a method for the detection of taurine content in eggs by high performance liquid chromatography（HPLC）,and taurine content of A and B brand dark eggs,C and D brand native eggs or ordinary eggs yolk was analyzed and compared,provided theoretical and practical reference for people to choose and consume eggs.In order to select the appropriate feed additives,the taurine content in A and B brand Porphyra yezoensis and C and D brand Porphyra haitanensis was compared and analyzed by HPLC.On this basis,taking shandong wenshang Gallus domestiaus as the test object,and added different concentrations of Porphyra,methionine and cysteamine into the diet respectively.Analysis of taurine content,egg quality index and egg production in yolk.The suitable levels of Porphyra,methionine and cysteamine were discussed to provide theoretical reference and practical basis for poultry industry to produce taurine-rich eggs.Text（1）Study on the determination of taurine content in 5 commercially available eggs by HPLCIn the test,HPLC autosampler with online derivatization function,pre-column derivatization with o-phthalaldehyde as derivatizing agent.Methanol+acetonitrile:water（25:75,v/v）was used as the mobile phase,the flow rate was 0.8 m L/min,and the column temperature was 30℃.Configure fluorescence detector for detection,excitation wavelength was 330nm,emission wavelength was 530nm.The results showed good linearity in the concentration range of 1 to 25μg/m L（R~2=0.9997）.The limit of detection（LOD）was 0.01μg/m L（S/N=3）,the recovery range was 99.7%to 100.5%,and the RSD range was 0.5%to0.6%.The taurine content of dark eggs of brands A and B,native eggs of brands C and D and ordinary eggs were determined by this method,and the taurine content was 1.332,1.151,1.063,0.735 and 0.622 mg/100 g.The taurine content in the yolk of ordinary eggs was significantly lower than that two kinds of brands dark eggs and C brands native eggs（P<0.05）,while the content of taurine in the yolk of D brand native eggs was slightly higher than that of ordinary eggs,but the difference was not significant（P>0.05）.Text（2）Study on the determination of taurine content in 4 commercial porphyra by HPLCThe Porphyra samples were crushed,stirred with water,and extracted in a thermostat water bath at 80℃.The gauze was filtered,centrifugated,and the supernatant was retained.Taken 3 m L Porphyra extract and adjusted p H to neutral.OPA was used as the derivating agent,and detect by HPLC-fluorescent detection（excitation wavelength 335 nm,Emission wavelength 530 nm）.In the range of 1-200μg/m L,R~2=0.9999,which indicates that the taurine standard solution has good linearity in this concentration range.When the taurine standard solution was added at 5,10,20μg/m L,the recovery range was between 95.2%and 104.9%,the relative standard deviation range was between 2.2%and 3.2%,and the detection limit was 0.01.μg/m L.This method was used to determine the A and B branded Porphyra yezoensis and C and D brand Porphyra haitanensis,and their taurine contents were 477.281,429.088,252.308,and340.546 mg/100 g.HPLC was accurate,simple,sensitive,fast,good reproducibility and selectivity,mild reaction condition,friendly to environment,the method could meet the test requirements of the taurine content in the Porphyra,Porphyra yezoensis of taurine content was significantly higher than the Porphyra haitanensis（P<0.05）,and the different brand Porphyra haitanensis taurine content was significant difference（P<0.05）.Text（3）Effects of adding Porphyra in daily ration on taurine content,egg quality and egg production in Gallus domestiaus yolkA total of 180 healthy and 26 weeks Gallus domestiaus were randomly divided into control group,3%Porphyra group,6%Porphyra group and 9%Porphyra group.Each group of 45,they were raised indoor flat areas,each group of 50 m~2 activity area,free access to food and water,pre-feeding for 3 days,formal test for 30 days.Around 10,20,and 30 days after the start of the formal test,15 eggs were randomly selected from each group for the determination and analysis of egg quality indicators,and 20 eggs were randomly selected from each group for the determination and analysis of taurine content changes in yolk by HPLC.The results show:on the 10th and 20th days,the taurine content in the yolk of the 9%Porphyra group was significantly higher than that in the 3%Porphyra group and the control group（P<0.05）;on the 30th day,the taurine content in the yolk of the test group was significantly higher than that of the control group（P<0.05）,and the content of taurine in yolk in the 9%Porphyra group was the highest,reaching 0.642 mg/100 g.On the 10th day,the egg weight of the control group was significantly higher than that of the test group（P<0.05）,the egg white ratio of the 6%Porphyra group and 9%Porphyra group was significantly higher than that of the control group（P<0.05）,the egg white weight of the control group was significantly higher than that of all the test groups（P<0.05）,and the haugh unit of the control group was significantly higher than that of the 6%Porphyra group（P<0.05）.On the 20th day,the egg white ratio of 6%Porphyra group and 9%Porphyra group was significantly higher than that of the control group（P<0.05）.On the 30th day,the eggshell weight of the control group was significantly higher than 3%Porphyra group and 6%Porphyra group（P<0.05）,and the eggshell ratio of the control group was significantly higher than 3%Porphyra group（P<0.05）.On the 10th day,the egg production of 6%Porphyra group and 9%Porphyra group was significantly lower than the control group,and the egg production of the 3%Porphyra group was significantly higher than that of the 9%Porphyra group（P<0.05）;On the 20th day,egg production in the control group and the 6%Porphyra group was significantly higher than that in the 3%Porphyra group and the 9%Porphyra group（P<0.05）.On the 30th day,the egg production of the control group was significantly higher than all the test groups,and the 3%Porphyra group was significantly higher than the 6%Porphyra Group and 9%Porphyra group（P<0.05）.The results indicated that adding a certain concentration of Porphyra to the daily ration could significantly increase the taurine content in the yolk,which had a certain effect on egg weight,shell weight,shell ratio and other egg quality indicators,and would result in a significant decrease in egg production.Text（4）Effects of daily ration methionine and cysteamine addition on taurine content,egg quality,and egg production in Gallus domestiaus yolkThe control group was fed a basic daily ration,the low concentration group was fed with0.2%methionine and 0.2%cysteamine,the medium concentration group was fed with 0.6%methionine and 0.2%cysteamine,and the high concentration group was fed with 0.9%methionine and 0.2 cysteamine.Other test designs,test animals and sample collection are the same as the test（3）.The results showed that the taurine content in the yolk of the three test groups was significantly higher than the control group（P<0.05）.The yolk of taurine content in the three test groups increased with the increase of the amount of the test.The taurine content in the yolk of the high concentration group was the highest at the 30th day,reaching 0.749 mg/100 g.On the 10th day,the control group of the egg weight was significantly higher than that of 0.9%methionine and 0.2%cysteamine group（P<0.05）,the yolk weight of 0.2%methionine and 0.2%cysteamine group was significantly higher than that of 0.6%methionine and 0.2%cysteamine group,and 0.9%methionine and 0.2%cysteamine group（P<0.05）,and the yolk ratio of 0.2%methionine and 0.2%cysteamine group was significantly higher than that of the control group and 0.6%methionine and 0.2%cysteamine group（P<0.05）;On the 20th day,the yolk color of0.9%methionine and 0.2%cysteamine group was significantly higher than that of the control group（P<0.05）,the egg white weight of 0.6%methionine and 0.2%cysteamine group was significantly higher than that of the control group（P<0.05）,the haugh unit of 0.2%methionine and 0.2%cysteamine group,and 0.6%methionine and 0.2%cysteamine group were significantly higher than 0.9%methionine and 0.2%cysteamine（P<0.05）,and the eggshell thickness of 0.9%methionine and 0.2%cysteamine group was significantly higher than that of the control group and 0.2%methionine and 0.2%cysteamine group（P<0.05）;On the 30th day,the yolk weight of 0.2%methionine and 0.2%cysteamine group was significantly higher than that of 0.6%methionine and 0.2%cysteamine group and high concentration group（P<0.05）,the yolk ratio of 0.2%methionine and 0.2%cysteamine group was significantly higher than the control,and 0.9%methionine and 0.2%cysteamine groups（P<0.05）,and the eggshell weight in 0.2%methionine and 0.2%cysteamine group was significantly higher than that in 0.6%methionine and 0.2%cysteamine group（P<0.05）.On the 10th day,the egg production of 0.2%methionine and 0.2%cysteamine group was significantly higher than that of the control group,0.6%methionine and 0.2%cysteamine group,and 0.9%methionine and 0.2%cysteamine group,and the egg production of the control and 0.6%methionine and 0.2%cysteamine group was significantly higher than that of 0.9%methionine and 0.2%cysteamine group（P<0.05）;On the 20th day,the egg production of 0.2%methionine and 0.2%cysteamine group and 0.6%methionine and 0.2%cysteamine group was significantly higher than that of the control group and 0.9%methionine and 0.2%cysteamine group（P<0.05）;On the 30th day,the egg production of the control group was significantly higher than that all of the test groups,and the egg production of 0.9%methionine and 0.2%cysteamine group was significantly higher than that of 0.2%methionine and 0.2%cysteamine group and 0.6%methionine and 0.2%cysteamine group（P<0.05）.The results suggest that adding certain concentrations of methionine and cysteamine in the daily ration can significantly increase the content of taurine in yolk,and have certain effects on egg quality indicators such as egg weight,yolk color,yolk weight,and eggshell weight.The egg production was increased in the early and middle stages of the low-concentration test,egg production decreased significantly of all the test groups in the later period.