| Blueberry is an emerging healthy fruit,which belongs to the fruit industry with high investment and high benefit,and has broad development prospect.One of the"bottleneck"problems in the development of the blueberry industry in shannxi is the lack of good plants of suitable blueberry cultivar.In the experiment,shaanxi suitable blueberry cultivar were selected as materials:the northern highbush blueberry cultivar"Brigita Blue","Legacy"and"Duke";the southern highbush blueberry cultivar"Emerald","Bluerain","Camille","Star","Misty","Farthing";the rabbit Eye blueberry cultivar"Brightwell"and"Baldwin".In order to establish an efficient and low-cost rapid propagation system of blueberry in tissue culture,and provide technical support and theoretical reference for the production of suitable cultivar of blueberry plants in Shaanxi province,research on tissue culture and rapid propagation technology of blueberry and optimization system for transplanting and domestication were studied.The main conclusions are as follows:1.Establishment of the first-generation aseptic system of blueberries.The results showed as follows:（1）Spring shoots can be carried out from April to May every year,The contamination rate of explants collected in May was lower and the proliferation rate was the highest;（2）In the primary culture,the middle-lower part,and lower part of the spring shoots are useful explants;（3）1/2 WM+1.0 mg·L-1 ZT was the best medium for primary culture..2.Subculture of blueberry.The results showed as follows:（1）Added in ZT and tZR was beneficial to the proliferation and growth of blueberry stem segments,but there were differences among different cultivar."Brigita Blue"and"Brightwell"had better proliferation effects in the medium supplemented with tZR."Legacy"grows well in ZT added medium;（2）The proliferation effect of"Brightwell"was the best when the tZR concentration was 2.0mg·L-1;（3）Different cultivar of blueberry had different adaptability to pH."Star"grew best at pH 4.5 and pH 5.0."Brigita Blue"grows best at pH of 5.5;（4）Added absorbent cotton and agar as the common support of medium was beneficial to the proliferation and cultivation of the blueberry variety"Brigita Blue".The blueberry cultivar"Brightwell"and"Legacy"had better proliferation effect on agar medium,and the cost of proliferation culture was lower.3.Stage of rooting culture in blueberry bottle.The results showed that/as follows:（1）The optimal rooting medium for different blueberry cultivar was different."Bluerain"could root efficiently in WPM and 1/2 WPM medium,and the rooting rate reached 100%in WPM+2.0mg·L-1 IBA medium.The best rooting medium for"Camille"was 1/2 WM+2.0 mg·L-1 IBA.1/2WPM+0.2 mg·L-1 IBA was the best rooting medium for"Brightwell";（2）The degree of rooting difficulty of tissue culture plantlet of different blueberry cultivar was different in the same medium.Different blueberry cultivar have different degrees of rooting difficulty in the same medium.In 1/2 WPM+2.0 mg·L-1 IBA medium,the rooting rate of"Bluerain"reached 91.99%,and the rooting time was short.The rooting time of"Brigita Blue"was long,it is not easy to take root,and the rooting rate was lower,only 11.11%.4.Transplantation and domestication of blueberry root tissue culture plantlets.The results showed that/as follows:（1）When blueberry rooted plants were domesticated and transplanted,wrapping moss on the roots and irrigating compound bacillus solution after transplanting helped to improve the survival rate of transplanting;（2）Using perlite+sawdust+peat（volume ratio1:2:1）and sand+clay+sawdust+peat（volume ratio 1:1:1:1）as transplanting substrate,blueberry plants grew vigorously;（3）When the blueberry plants are directly applied with cake fertilizer,10 g per pot can promote the growth of the plants.If the direct application amount is too large,the plants will grow slowly and even die;（4）1/2 WM nutrient solution is beneficial to the growth of blueberry plants（pH 4.3～4.8）,and the plants grow robustly. |
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