Genetic Differentiation,Host Adaptation And Main Control Techniques Of Tuta Absluta(Meyrick)

As one of the world’s quarantine pests,tomato leaf miner mainly harms Solanaceae plants,especially the host plant tomato.In recent years,due to the invasion of this pest in my country,the development of tomato-related industries has been seriously hindered.In order to prevent the further spread and spread of the tomato leaf miner in Xinjiang,this study based on ISSR molecular marker method to study the genetic diversity of the tomato leaf miner,and to carry out its adaptability to common host plants and main control techniques.etc.tests.The main conclusions are as follows:1.In this study,15 polymorphic primers were screened out,and a total of 137 ISSR specific bands were amplified,of which polymorphic bands accounted for 96.35%,and all individuals showed their own unique ISSR patterns.The results of ISSR marker genetic diversity showed that the genetic distances of the 20 tomato leafminer geographic populations ranged from 0.0065 to0.1623,and the genetic consistency ranged from 0.8502 to 0.9935.According to the analysis of the source of population variation,74.64%of the variation came from within the population.The UPGMA phylogenetic analysis showed that there was no strong correlation between the clustering of each geographic population and the geographic location.The results of the ISSR study showed that the tomato leafminer is still in the early stage of invasion,and has the characteristics of frequent invasion and multiple spots.2.The growth and development of tomato leafminers on 13 host plants including Solanum lycopersicum、Solanum nigrum L.、Lycium barbarum L.、Convolvulus arvensis L.、Chenopodium hybridum L.、Chenopodium glaucum L.、Spinacia oleracea L.、Solanum melongena L.、Lactuca sativa var.Ramosa Hort.、Preserved vegetable、Vigna unguiculata(Linn.)Walp.、Cucumis sativus L.、Helianthus annuus L.were studied.Population growth parameters and effects of spawning selection.The results showed that the adults’preference for 13 host plants for spawning was S.lycopersicum>S.nigrum L.>S.melongena L.>L.barbarum L.>C.sativus L.>Preserved vegetable>C.arvensis L.>H.annuus L.>C.glaucum L.>V.unguiculata(Linn.)Walp.>C.hybridum L.、S.oleracea L.and L.sativa var.Ramosa Hort..The eggs laid on Preserved vegetable.have the longest development period,and the larvae cannot complete life histiry after hatching.The pre-adult survival rate on S.lycopersicum was higher than that of other host plants,but the total development period was longer.The egg production and female lifespan after feeding on S.lycopersicum leaves were higher than those of other host plants.The intrinsic growth rates of the S.lycopersicum、S.nigrum L.、S.melongena L.、L.barbarum L.leaves were 0.1402,0.1139,0.1100,and 0.0797,respectively.In general,S.lycopersicum is the most suitable host for the tomato leaf miner;life cycle cannot be completed on the C.arvensis L.、C.hybridum L.、C.glaucum L.、S.oleracea L.、L.sativa var.Ramosa Hort.、Preserved vegetable、V.unguiculata(Linn.)Walp.、C.sativus L.、H.annuus L.;The population growth potential of S.nigrum L.、S.melongena L.、L.barbarum L.is high,and the tomato leaf miner has the potential to explode into disasters on these three host plants.3.In order to effectively prevent and control the damage of tomato leaf miners,the screening test of pesticide was carried out,and the best control chemicals and application concentrations were determined.10 insecticides were selected,and 26 chemical treatments and 1 clear water treatment were set up as control.On the 3rd,7th,10th,and 14th days after the application of the pesticides,the number of insect populations on the leaves was investigated,and the reduction rate of the insect population and the correction control were analyzed.The results showed that,the combination of 34%ethoxylate SC 48 ml/667 m~2,6%spinosad SC 50 ml/667 m~2and 0.05%silicone additive 15 ml/667 m~2,6%Spinosad SC 50 ml/667 m~2and 20%Chlorantraniliprole SC6.7 ml/667 m~2,20%Chlorantraniliprole SC 10 ml/667 m~2at the 10th days after treatment,the field control effect can reach 98.33%,99.24%,96.68%and 90.15%in the field test;After 14 days,the field control effect can reach 94.59%,93.10%,90.97%and 91.29%respectively.In general,the pesticides mentioned above can be used as pesticides for the control of tomato leaf miners.This experiment provides a certain scientific reference for the study of chemical control and comprehensive management of tomato leaf miners.4.By setting serial levels of tomato leafminer egg density,temperature,humidity,presence or absence of light and the production days of tomato leafminer eggs,the main factors affecting the parasitism of Trichogramma pintoi tomato leafminer eggs were evaluated.The parasitic rate of single female T.pintoi was 30 eggs/tube,the highest parasitic rate was 93.33%under the conditions of temperature of 26℃,relative humidity of 75%and darkness.The longer the egg production time of tomato leaf miner,the weaker the parasitic ability of T.pintoi.According to the fitting results,the parasitism of T.pintoi on tomato leafminer eggs is consistent with the disc equation of the Holling-Ⅱtype functional response,indicating that the release of T.pintoi in the field may become an effective biological control method for the control of tomato leaf miners.