| At present,there have been some reports on the research of the application effect of the white wine grain yeast culture in the fattening pigs,but there are not many studies on the application effect of weaned piglets.Therefore,the purpose of this article is to explore the application and evaluation of the addition of white lees Saccharomyces cerevisiae culture(SDGS)to weaned piglets,and to provide a theoretical basis and scientific basis for the development and utilization of unconventional feed materials and healthy green efficient breeding.In this experiment,12 21-day-old weaned piglets with similar body weight and good health were pre-fed with a basic diet for 3 days,and were randomly divided into 2 groups to ensure that there was no significant difference in body weight between each group.Each group had 6 replicates.The experimental group added 4% SDGS to the basic diet,and the control group was fed the basic diet for 28 days.Piglet feeding management is the same as pig farm standardization management.The test pigs were free to eat and drink water,and the weight and feed intake of the piglets were weighed at the beginning and end of the experiment,respectively.To explore the effects of SDGS on growth performance and organ index,serum biochemical and immunoglobulin,intestinal health and intestinal microbes of weaned piglets.The results were as follows:(1)the effect of SDGS on the growth performance of weaned piglets: compared with the control group,4% sdgs could significantly improve the average daily gain(P < 0.05),and the daily intake and feed weight ratio had no significant effect.(2)The effect of SDGS on organ index of weaned piglets: compared with con,4% sdgs significantly reduced the organ index of liver(P < 0.05),and there was no difference in organ index of heart,spleen,spleen,pancreas and kidney.(3)The effect of SDGS on serum biochemical and immunoglobulin indexes of weaned piglets:compared with the control group,4% sdgs had no significant effect on serum biochemical indexes of weaned piglets.However,there was a trend of increasing serum TP and decreasing urea and glucose levels in the diets supplemented with 4% sdgs(P > 0.05),while Ig G and Ig M increased slightly in the diets supplemented with 4% sdgs(P > 0.05).(4)The effect of SDGS on the intestinal health of weaned piglets: 1)compared with the control group,adding 4% sdgs diet had no significant effect on the height of villus,the depth of crypt and its ratio(villus height / crypt depth,V / C)(P > 0.05),but significantly reduced the height of villus of jejunum(P < 0.05),significantly increased the depth of crypt of jejunum(P < 0.05),and significantly reduced the V / C of jejunum(At the same time,it significantly increased the depth of ileal crypt(P < 0.05),and significantly reduced the V / C of ileum(P < 0.01).2)The results showed that there was no significant effect of 4% sdgs on the relative expression of Claudin-3,occludin and ZO-1 mRNA in duodenum,jejunum and ileum of weaned piglets(P > 0.05).3)Compared with the control group,the level of IL-6 mRNA expression in duodenum and ileum mucosa was significantly reduced by adding 4% sdgs(P < 0.05),while the level of IL-6,IL-1 β and IL-10 mRNA expression in jejunum mucosa was not significantly affected(P > 0.05).There was no significant effect on the mRNA expression of IL-1 β and IL-10 in duodenum and ileum(P > 0.05).(5)The effect of SDGS on the intestinal microflora of weaned piglets: 1)compared with con group,SDGS increased the observed species and Shannon index(P < 0.05)of jejunal microflora of weaned piglets,and affected the richness of jejunal microflora;decreased the Chao1 and ACE index of ileal microflora of weaned piglets,and affected the richness of ileal microflora.There was no significant difference in microbial diversity between the cecum and the colon.2)The results of principal component analysis(PCo A)showed that the distribution of β diversity in jejunum of con group and sdgs group was scattered,and the distance was far,which indicated that their microbial community structure was quite different.In cecum,con group and SDGS group were grouped into one group,but there was also a trend of separation,indicating that there was also a certain difference in microbial community structure.The species complexity of jejunum and ileum in weaned piglets was significantly improved by adding SDGS.3)The SDGS group and the control group ranked the top 10 species in terms of microbial abundance of jejunum,ileum,cecum and colon contents relative to colony gate level.The results showed that in jejunum of weaned piglets,Firmicutes,Bacteroidetes,Proteobacteria and tenericetes were the main bacteria of each group;in ileum,Firmicutes and Proteobacteria were the main bacteria of each group;in cecum and colon,Firmicutes,Bacteroidetes,Proteobacteria and spirochaetes were the main bacteria of each group.4)The sequence of bacteria in jejunum,ileum,cecum and colon was analyzed by nonparametric statistical method at the level of phylum and genus.The results showed that there was no significant difference in the flora at the gate level.At the Order level,SDGS group significantly increased selenium(P < 0.05)and lactobacillales(P < 0.05)in jejunum;at the generic level,SDGS group significantly increased blautia(P < 0.05),holdemanella(P < 0.05),phascolarctobacterium(P = 0.044),ruminoccus-1(P = 0.034),ruminocaceae UCG in jejunum-010(P < 0.05),lachnospiraceae ucg-010(P = 0.029),lachnospiraceae ucg-010(P < 0.05),adding sdgs to the diet significantly reduced the ileum clostridium-sensu-stricto-1(P < 0.05),but significantly increased terrisporobacter(P < 0.05);adding SDGS to the diet significantly increased the cecum Lactobacillus(P < 0.05),lachnospiraceae ucg-010(P < 0.05),but significantly In addition,dietary SDGS significantly reduced colon solobacterium(P < 0.05)and ruminocaceae-5(P < 0.05).In summary,it is concluded that the Saccharomyces cerevisiae culture using Distillers’ Grains can be used as an unconventional feed additive for weaned piglets.4% sdgs significantly increased the average daily gain(P < 0.05)and decreased the feed to weight ratio(0.05 < P < 0.1),which played a positive role in maintaining intestinal microecological balance and promoting intestinal health. |
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