Regulation Of Four Environmental Factors On Color Physiological Characteristics Of Acer Palmatum Thunb. And Acer Palmatum ’Atropurpureum’

The colorful leaf forest is one of the key ecological system types unique to Southwest China.It not only has important ecological functions such as water conservation,soil and water conservation and climate regulation,but also has significant landscape aesthetic value.However,continuous human activities,frequent natural disasters,climate change,and their superimposed effects have resulted in a single composition of large-scale colorful leaf forests,simplified structures,and continuous decline or even disappearance of ecological services such as landscape aesthetics.Therefore,the restoration and reconstruction of colorful-leaved forest landscapes are playing an increasingly important supporting role in the development of characteristic ecotourism and the realization of regional economic and social sustainable development.This paper aims at the insufficient technical reserves of restoration and reconstruction of degraded colorful leaf forest landscape,and it is difficult to effectively serve the restoration and reconstruction of degraded ecosystems.In this area,the collection and preservation of color leaf plant resources are insufficient,and the technical system of color leaf plant domestication and fast growing cultivation is seriously lacking.Studies on the regulation of four environmental factors on the color physiological characteristics of two color leaf plants are carried out.In this paper,the physiological studies on the color transformation stage of two maple plants,Acer palmatum Thunb.and Acer palmatum’Atropurpureum’were carried out,and the effects of different light intensity,soil alkalinity,Cu²⁺ and Fe²⁺ concentration on the color rendering of the two plants in autumn were discussed.This experiment was conducted in June2018 at Xuecheng Forest Farm,Xuecheng Town,Li County,Aba Tibetan and Qiang Autonomous Prefecture,Sichuan Province.Until November 2019,the experiment had 4treatment factors: light intensity test group,using 100%（blank control）,80% And 50% light intensity;Soil p H test group was treated with clear water,p H6 and p H4 citric acid buffer;Cu²⁺ test group was treated with clear water,0.3% Cu SO₄ and 0.6% Cu SO₄ respectively Application;Fe²⁺ test group,using clean water,0.3% Fe SO₄ and 0.6% Fe SO₄ for foliar spray.The experiment determined the chlorophyll a,chlorophyll b,carotenoids,anthocyanins,chlorophyllase,phenylalanine ammonia-lyase,peroxidase,superoxide disproportionation during the discoloration period of Acer palmatum Thunb.and Acer palmatum’Atropurpureum’.Enzyme,soluble sugar,soluble protein,proline and malondialdehyde and other physiological indicators,the effects of four treatment factors on the physiological indicators during the color change of Acer palmatum Thunb.and Acer palmatum’Atropurpureum’ were analyzed,so as to select the most suitable cultivation conditions,Provide a theoretical basis for the introduction and management of 2 species of Aceraceae plants,restoration of colored leaf forest.The results showed that the content of chlorophyll and carotene in Acer palmatum Thunb.and Acer palmatum’Atropurpureum’ decreased with the change of leaf color under each treatment（P <0.05）,and the content of anthocyanin and malondialdehyde increased significantly.The specific effects of different treatments were different :（1）Under different light intensity treatments,as the light intensity decreases,the chlorophyll content,chlorophyllase activity,and phenylalanine ammonia lyase activity decrease significantly.Anthocyanins also decrease significantly in late autumn and early winter,but peroxidase instead The activity of relative control is enhanced;the content of soluble sugar is the highest at 80% light intensity,and the change of proline is not significant in 2019;（2）Soil p H treatment,appropriate reduction of soil alkalinity,chlorophyll content is significantly reduced,anthocyanin content is significantly increased,Chlorophyllase activity in the p H4 citrate buffer treatment group was significantly lower than the control,superoxide dismutase activity was significantly higher than the control,Acer palmatum Thunb.phenylalanine ammonia lyase September 2018 p H4 citrate buffer treatment group activity Significantly lower than the control,the Acer palmatum’Atropurpureum’ soluble protein in the p H6 citrate buffer treatment group in 2019 was significantly higher than the control;（3）With different Fe²⁺ and Cu²⁺concentration treatments,the content of chlorophyll a in Acer palmatum’Atropurpureum’ is significantly different from that of the control.Two samplings in 2018,the content of anthocyanin in Acer palmatum Thunb.at 0.6% concentration was significantly higher than that of the control,and the anthocyanidin when the leaves were red The glycoside content is lower than the control.With Cu²⁺ treatment,chlorophyllase activity decreased with increasing Cu²⁺ concentration,peroxidase activity and soluble protein content did not change significantly,superoxide dismutase activity increased,and the malondialdehyde content of 0.6%Cu²⁺ treatment group was significant Higher than the control;Fe²⁺ treatment,the chlorophyllase activity of the 0.6% Fe²⁺ group was significantly lower than that of the control,the peroxidase activity of Chinese red maple was the strongest,the chlorophyllase activity of the 0.3% Fe²⁺ group was the strongest,the soluble protein content increased with the concentration of Fe²⁺ Increase.In general,Acer palmatum Thunb.and Acer palmatum’Atropurpureum’ have brighter leaf colors and higher ornamental value when the light intensity is increased and the soil alkalinity is appropriately reduced,while Cu SO₄ and Fe SO₄ can discolor the two maple plants in advance The final leaf color is not as bright as the control,and Cu SO₄ and Fe SO₄ are not suitable for late autumn.In future research,we can study how these test factors affect the gene expression of plants in terms of gene expression,thereby affecting plant physiological substances,and ultimately regulating the color change of plant leaves,to fundamentally grasp that plants are regulated by different environmental factors Mechanisms.