| Dairy cow mastitis has become a common problem that needs to be solved in the dairy farming process.It not only restricts the development of dairy industry,but also one of the most important factors that endanger the health of consumers.Due to the many induced factors(season,age,parity,lactation stage,environmental health,etc.)and the complex pathogens(with regional characteristics),it also brings great challenges and difficulties to the treatment and prevention.In Yanqi county of Bayinguoleng Mongol Autonomous Prefecture Region from Xinjiang,the prevalence and occurrence of dairy cow mastitis is relatively common,and there are few research reports on the epidemiological investigation of dairy cow mastitis in this area.In order to understand the etiology of cow mastitis in this region and the drug resistance of main pathogens,this study took dairy cows as the research object in Yanqi county of Bayinguoleng Mongol Autonomous Prefecture Region from Xinjiang.BMT,pH value and somatic cell count test were combined to diagnose the incidence of recessive mastitis.The parity,age and season of cattle were investigated and analyzed was carried out.Routine microbiological methods(Isolation culture,biochemical identification,etc)were used to isolate and identify pathogenic bacteria and test the drug sensitivity of milk samples.Relevant test results are as follows:1.An epidemiological investigation of cow mastitis in Yanqi county of Bayinguoleng Mongol Autonomous Prefecture Region from Xinjiang showed that there were 352 cow with clinical mastitis,accounting for 8.76%(352/4016)of the tested cows.There were 973 cows with subclinical mastitis,accounting for 34.44%(973/2825),among which 1725 cows with positive milk area,accounting for 31.22%(1725/5526).Statistical analysis of the incidence of cow mastitis and milk area,age,parity and season showed that the incidence of cow mastitis was the same in different milk areas,but with the increase of age and parity,the incidence was on the rise.It is a typical seasonal disease with a high incidence in winter and spring.2.A total of 207 strains of 14 species of pathogenic bacteria were isolated from 352 cows with clinical mastitis from 4016 cows,including 62 strains of Staphylococcus(accounting for 29.95%),69 strains of Streptococcus(accounting for 33.33%),42 strains of Escherichia coli(accounting for 20.29%).A total of 871 strains of 14 species of pathogenic bacteria were isolated from 973 cows with subclinical mastitis from 2825 cows,including 229 strains of Staphylococcus(accounting for 26.29%),385 strains of Streptococcus(accounting for 44.20%),254 strains of Gram-negative bacilli(accounting for 29.16%),including 114 strains of Escherichia coli(accounting for 13.09%).The mastitis that causes by Staphylococcus,Streptococcus and Escherichia coli among them accounts for 83.6%of total number.3.Twelve drug susceptibility tests were conducted on 113 strains of causing cow clinical mastitis,which showed high resistance to ampicillin,amoxicillin,cefrazine,cefotaxime and cefoxitin(52.31%-62.83%),and high sensitivity to gentamicin,cotrimoxazole and chloramphenicol(53.98%-67.26%).It is suggested that the traditional treatment of cow mastitis in this area cannot be used,and targeted treatment and prevention should be carried out according to the situation of drug use in this area.The incidence of clinical mastitis was 8.76%and that of subclinical mastitis was 34.44%in dairy cows in Yanqi county of Bayinguoleng Mongol Autonomous Prefecture Region from Xinjiang.A total of 14 species of 1078 strains were isolated,of which 83.6%were caused by Staphylococcus,Streptococcus and Escherichia coli.There was a high resistance to ampicillin,amoxicillin,cefrazine,cefotaxime and cefoxitin,and a high sensitivity to cotrimoxazole and chloramphenicol,which provided certain data support for the treatment of cow mastitis in this area. |
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