Study On The Effect Of Royal Jelly And Chitosan On The Quality Of Semen Preservation In Experimental Beagle Dogs

The preservation of semen is of great practical importance as it can realize the transportation of semen from one place to another and ensure the reasonable cross-regional utilization of germplasm resources.In this study,Uppsala diluent was used as the base diluent for the cryopreservation and cryopreservation of semen,and different concentrations of royal jelly and chitosan were added to the diluent,respectively.The optimal addition was screened by detecting the levels of sperm viability,viability,plasma membrane integrity,acrosome integrity,malondialdehyde(MDA)level,catalase(CAT)level and total antioxidant capacity after preservation The optimal concentrations of royal jelly and chitosan were added to the dilutions,and their effects on semen preservation were verified by artificial insemination and the determination of mRNA levels of related genes.The results of the study were as follows:Translated with www.Deep L.com/Translator(free version)1.During the low temperature(4°C)preservation of canine semen,the sperm viability and sperm viability were not significantly different from the control group when royal jelly was added at a concentration of 0.8%;acrosome integrity,plasma membrane integrity,catalase(CAT)level and total antioxidant capacity were significantly increased,and malondialdehyde(MDA)level was significantly decreased(P < 0.05);the sperm viability,sperm viability,plasma membrane integrity,catalase(CAT)level and total antioxidant capacity were not significantly different from the control group when chitosan was added at a concentration of 0.8 mg/ml.The sperm viability,acrosome intactness,plasma membrane intactness,catalase(CAT)level and total antioxidant capacity were significantly increased and malondialdehyde(MDA)level was significantly decreased(P< 0.05);the conception rate,litter size and litter growth after artificial insemination were not significantly different from the control group.2.During frozen(-196°C)storage,the sperm viability after thawing was not significantly different from that of the control group when royal jelly was added at a concentration of 0.8%.The sperm viability,acrosome integrity,plasma membrane integrity,total antioxidant capacity of catalase(CAT)level were significantly increased and malondialdehyde(MDA)level was significantly decreased(P < 0.05);the sperm viability after thawing was not significantly different from that of the control group when the concentration of chitosan was 0.8 mg/ml.The sperm viability,acrosome integrity,plasma membrane integrity,total antioxidant capacity of catalase(CAT)level were significantly increased and malondialdehyde(MDA)level was significantly decreased(P < 0.05);the fertilization rate was lower after artificial insemination,and the fertilization rate,litter size and litter growth were not significantly different from the control group.3.The relative expression of ROS regulatory factor 1(ROMO1)mRNA was not significantly different from the control group after 1 week of cryopreservation and freezing with optimal concentrations of royal jelly and chitosan alone;the ratio of the relative mRNA expression of apoptosis-related genes B-cell lymphoma-2(Bcl-2)and Bcl-2-associated X protein(Bax)was significantly higher;sperm acrosome associated protein-3(SPACA3)mRNA relative expression was significantly increased(P < 0.05).