Studies On Differential Proteomics And Metabolomics Of NLEA1 Transgenic Wheat

The salinization of the soil will have a significant impact on the growth and development of plants,and is a major concern for many experts and scholars.Because the research of soil salinization is directly related to the sustainability and rationality of soil development and utilization,it is of great importance.Theoretical and practical significance,NLEA1 gene is a novel gene selected and synthesized by bioinformatics software for the hydrophilic short peptide sequence of plant LEAs protein database,which plays an important role in the salt tolerance of Arabidopsis,wheat and yeast effect.In this paper,the leaves of wheat transformed with the NLEA1 gene and the control Fielder variety were used as materials,using 200 mmol/L Na Cl salt stress and non-salt stress treatment.For proteomics,the gel after2-DE electrophoresis was performed by Image Master 2D Platinum 5.0 software,The analysis found that Fielder had 121 protein sites under non-salt stress and 177 protein sites under 200mmol/L Na Cl salt stress treatment;wheat with NLEA1 gene had 165 protein sites under non-salt stress.The largest number of protein is 219 protein sites under 200mmol/L Na Cl salt stress treatment,.By comparing the experimental treatments in pairs,select a comparison group with a magnification more than 4,the dots are clear and do not adhere to other protein spots.a total of 16 differential protein spots with round glue dots,which are convenient for mass spectrometry identification,are identified by mass spectrometry.Among them,13 differential protein spots were successfully identified.These differential protein spots were:photosystem I iron-sulfur center,L-lactate dehydrogenase,LMWPc structural protein,panthenol oxidase,glutamate dehydrogenase,protein kinase Binding protein,E3 ubiquitin protein ligase,ribose diphosphate carboxylase,serine/threonine protein kinase receptor,cytokinin oxidase/dehydrogenase 2,PPR-long domain protein,polyphenol oxidase,ammonia Methyltransferase.In metabolomics,GC-MS technology was used for qualitative and quantitative analysis of small molecule metabolites,and it was found that 11 different metabolites were screened out of 2 materials without salt stress treatment,including amino acids,organic acids and sugars The proportion of small molecular differential metabolites is equal to 27.27%,the differential metabolites of other substances account for 18.18%,a total of 29 differential metabolites were screened under 200 mmol/L Na Cl salt stress and non-salt stress.11 amino acid differential metabolites,accounting for a maximum of 37.93%,Secondly,there are 10 carbohydrate differential metabolites,accounting for 34.48%.Using Metabo Analyst 4.0 online analysis platform for metabolic pathway analysis,4 metabolic pathways related to transgenes were selected,and 5 differential metabolic pathways related to salt stress were selected.