Hormone Regulation On Vascular Bundle Development And Stone Cell Formation In Pear Fruit

In this study,100 different varieties of pear fruit were dyed by hand and stained with paraffin sections,the whole field scanning image technique was used to collect the dyed pictures of different varieties,which was explore the relationship between vascular bundle development and stone cell mass formation.In order to quantitatively analyze the relationship between vascular bundles and stone cells,this experiment used mathematical modeling methods to extract the characteristic morphology and color of vascular bundles and stone cells,and to accurately cluster the number and distribution of stone cell clusters.The distribution of stone cell masses provided a precise analytical method and also provided a theoretical basis for the relationship between stone cells and vascular bundles.In order to further explore whether vascular bundle development and stone cell formation are related to endogenous hormone regulation,the content of stone cells and endogenous hormones in different parts of the fruit were determined by field treatment experiments.At the same time,the hormone immunofluorescence localization of different treatments was compared with the field experiment results,and the localization results were consistent with the field treatment results.The indoor and outdoor hormones were used to treat the callus of the ’Dangshansuli’,so as to screen out the hormones that significantly affect the stone cells content.It provided theoretical guidance for inhibiting the content of stone cells in the fruit and improving the fruit quality through the cultivation measures of hormone treatment.The results of this test were as follows:1、The vascular bundles of 100 different varieties of pear fruit were distributed in a ring shape,and the vascular bundles near the core were evenly distributed and dense.The stone cells in different varieties are clustered near the vascular bundle and distributed around the vascular bundle.According to the modeling results,in the different varieties of pear fruit,the ratio of the number of stone cell clusters to the number of total stone cell clusters at 0.3 cm from the vascular bundle is between 70%and 80%.Among the average of 100 varieties,the number of stone cell clusters at 0.3 cm from the vascular bundle accounted for 79.84%of the total number of stone cell clusters.2、‘Dangshansuli’and‘Cuiguan’were collected as experimental species,the relationship between stone cell formation and hormone regulation was studied by measuring the hormone and stone cell content in different parts of the fruit.The results showed that in different treatments,the enrichment and non-enrichment zone of stone cells increased gradually in the early growth stage,but decreased in the fruit ripening period and late maturity.Studies have shown that the content of stone cells in the different treatments increased in the early stage and decreased in the later stage.The content of stone cells in different parts was related to the hormone content.GA3 inhibited the formation of stone cells 30 d after the flowering,and promoted the formation of stone cells from 30-50 d GA3.In the enrichment area‘Cuiguan’and the green bag‘Dangshansuli’,the content of stone cells is the lowest when the ZT content is the highest;‘Dangshansuli’and the orange bag ’Dangshansuli’ are the opposite.ZT in non-enriched areas promoted the increase of stone cell content 30-50 d after flowering.IAA inhibited the formation of stone cells in the first 30 d after flowering,from 30-50 d,there was a tendency to promote the formation of stone cells.3、Immunofluorescence localization indicated that GA3 and IAA were aggregated in the vicinity of vascular bundles in different treatments,and the orange bag was higher than the green bag.The green area and the strength of the GA3 around the vascular bundle and the stone cells were higher.The excitation intensity of ABA in the vicinity of different treatment vascular bundles was lower,and significantly lower than other hormones.The ABA content of vascular bundle transport was less,and the effect of other hormones on stone cell distribution was less significant.4、On the basis of field treatment experiments,this experiment verified the effects of different exogenous hormone treatments on the lignin content of callus in ’Dangshansuli’by isolated experiments.The results showed that the callus was expanded faster,had good growth and a moist surface,in which treated by exogenous gibberellin.The content of lignin in the callus decreased gradually with the increase of treatment time;appropriate exogenous auxin and cytokinin-treated callus have lower lignin content.However,the concentration of the callus was too high or too low,the surface of the callus was dry,browning,the lignin content was higher and the highest peak appeared on the 15th day;the callus after exogenous abscisic acid treatment had a dry and browning surface,and the lignin content increased first and then decreased with the treatment time.Comprehensive comparative analysis found that gibberellin had a significant effect on lignin synthesis.