| Bile acid is the main component of bile,which is synthesized from cholesterol in the liver,stored in the gallbladder,and then secreted into the duodenum through the bile duct.BA can promote the emulsification of fat and increase the absorption of fatty acids,it is widely used as a dietary additive in livestock and aquaculture.In addition,BA act as a signaling molecule decreasing serum lipids levels and mediating adipose tissue thermogenesis.However,it is still unclear whether using BA as a physiological regulator in pregnant sows could affect hepatic lipids and adipose tissue lipids metabolism in offspring piglets.The aim of this study was to investigate the effects of maternal bile acid supplementation on liver and adipose tissue lipid metabolism in offspring piglets.1 Effects of maternal dietary bile acid supplementation on hepatic lipids metabolism in offspring pigletsIn this experiment,thirty Landrace × Large white crossbreed sows were randomly divided into two groups at 90th day of gestation(15 in each group).Sows in the control group(Con)received basal diet whilst the experimental group(BA)were fed bile acid-supplemented(100 mg/kg)diet during gestation and lactation.The experiment was finished at 21 days lactation.When the experiment finished,one male piglet per litter close to the mean body weight of 7 litters in Con sows and 8 litters in BA sows was killed for sampling.Serum and liver samples were collected.It was shown that maternal bile acid supplementation significantly decreased the content of serum triglyceride(P<0.05),and serum non-esterified fatty acids concentration tended to decrease(P=0.06)in BA-treated offspring piglets.Maternal bile acid supplementation did not significantly affect hepatic total TG content.Western Blot was used to detected the protein content of lipogenesis,lipolysis,lipids transport and fatty acid β-oxidation related genes,the results showed that the protein content of liver X receptor was significantly decreased and Steroid response element binding protein 1 protein content tended to decrease(P=0.08)in BA-treated offspring piglets.These results suggested that maternal bile acid supplementation reduced serum triglyceride content,but did not significant affect hepatic lipids deposition and lipids metabolism in offspring piglets.2 Effects of maternal dietary bile acid supplementation on adipose tissue metabolism in offspring pigletsThe offspring piglets were killed.The back subcutaneous fat samples were collected and the back fat thickness was recorded.It was shown that maternal bile acid supplementation significantly decrease the adipocyte area(P<0.01).No significant alterations was detected for the back fat thickness(P>0.05).Fluorescence real-time quantitative PCR was used to determine the mRNA abundance of genes involved in lipids metabolism.The results shown that maternal bile acid supplementation significantly reduced the mRNA expression of acetyl-CoA carboxylase(P<0.05),stearoyl-CoA desaturase(P<0.05),perlipin1(P<0.05),perlipin3(P<0.05),perlipin4(P<0.05),Cell death inducing DFFA like effector a(P<0.05),CCAAT enhancer binding protein beta(P<0.05),adipose triglyceride lipase(P<0.05),hormone sensitive lipase(P<0.05),lipoprotein lipase(P<0.05)and CD36(P<0.05)in offspring piglets adipose tissue.However,no significant changes were detected for the adipose tissue protein content of lipogenesis,lipolysis and adipose tissue browning related-genes.In addition,we also detected mRNA abundance of mitochondrial DNA encoded genes.The results shown that maternal bile acid supplementation significantly decreased the mRNA expression of ND6(P<0.05),and the mRNA expression of ATP6(P=0.07)and ATP8(P=0.07)tended to decreased.Furthermore,maternal bile acid supplementation significantly decreased the adipose tissue contents of polyunsaturated fatty acid C20:3n-6(P<0.05),and the concentration of monounsaturated fatty acid C20:1 tended to decreased(P=0.09)in BA-treated offspring piglets.These results indicated that maternal bile acid supplementation significantly decreased the content of polyunsaturated fatty acid C20:3n-6 in the back fat of offspring piglets without on lipids metabolism process and adipose tissue browning.In summary,maternal bile acid supplementation significantly reduced the serum triglyceride content,the adipocyte area and the back subcutaneous fat polyunsaturated fatty acid C20:3n-6 content.However,no significant changes were detected for the lipids metabolism related-genes expression in liver and adipose tissue. |
PDF Full Text Request