Cloning And Expression Analysis Of Genes Related To Anthocyanins Of ‘Pingguoli’

Bagging treatment can make the fruit of ‘Pingguoli’ appear red and improve the appearance quality of the fruit.The degree of fruit coloration is affected by the anthocyanin content,but the biosynthesis of anthocyanins is regulated by the MYB transcription factor,bHLH and WD40 transcription factor.Related MYB,bHLH transcription factor play an important role in the regulation of anthocyanin biosynthesis.Through the preliminary research in this laboratory,it was found that MYB108,bHLH62,bHLH130 and MYC2 have certain effects on fruit coloring.In this experiment,’Yanbian Pingguoli’ was used as the test material.The genes PbMYB108,PbbHLH62,PbbHLH130,and PbMYC2 in the MYB and bHLH transcription factor families in ‘Pingguoli’ were cloned.Changes in expression and changes in anthocyanin content,and further explore the relationship between the above genes and anthocyanin biosynthesis.The main results are as follows:1.In this experiment,PbMYB108,PbbHLH62,PbbHLH130 and PbMYC2 genes were successfully cloned and bioinformatics analysis was performed on them.2.Using qRT-PCR method to measure the expression of the above genes in different periods of ’Pingguoli’ bagging and natural growth conditions.It was found that the expression of PbMYB108 and PbbHLH62 were not significantly correlated with anthocyanin accumulation.The expression of PbbHLH130 and PbMYC2 were significantly positively correlated with anthocyanin accumulation,suggesting that they were important genes in anthocyanin synthesis.3.Bagging treatment can improve the coloring of ‘Pingguoli’.Exposure after unpacking will significantly increase the production and accumulation of anthocyanins.The content of anthocyanins in ‘pingguoli’ fruits without bagging is low and stable.The chlorophyll content of bagged fruits showed a wave dynamic trend after unbagging,and was always lower than that of unbagged fruits.After bagging and unbagging,the carotenoid content in the fruit gradually increased,but it was always lower than that in the unbagged fruit.